Ekusesheni okuqhubekayo kwesidakamizwa se-antidiabetic esiphephile nesisebenzayo, ulwelwe lwasolwandle luba umthombo obalulekile ohlinzeka ngenhlanganisela yamandla amakhulu okwelapha.I-Alpha-amylase, i-alpha-glucosidase inhibitors, nama-antioxidant compounds ayaziwa ukuphatha isifo sikashukela futhi athole ukunakwa okukhulu muva nje.Esifundweni samanje, ama-algae amane aluhlaza (i-Chaetomorpha aerea, i-Enteromorpha intestinalis, i-Chlorodesmis, ne-Cladophora rupestris) yakhethwa ukuhlola i-alpha-amylase, i-alpha-glucosidase inhibitory, kanye nomsebenzi we-antioxidant ku-vitro. .Umsebenzi we-Antidiabetic wahlolwa ngamandla okuvimbela ama-extracts ngokumelene ne-alpha-amylase ne-alpha-glucosidase ngama-spectrophotometric assays.Umsebenzi we-Antioxidant unqunywe yi-2,2-diphenyl-1-picrylhydrazyl, i-hydrogen peroxide (H2O2), kanye ne-nitric oxide scavenging assay.Ukuhlaziywa kwe-gas chromatography-mass spectrometry (GC-MS) kwenziwa ukuze kutholwe isakhiwo esikhulu esinesibopho sesenzo saso se-antidiabetic.Phakathi kokukhishwa okuhlukahlukene okuhloliwe, ukukhishwa kwe-chloroform ye-C. aerea (IC50 - 408.9 μg/ml) kanye ne-methanol ekhishwe ku-Chlorodesmis. (IC50 - 147.6 μg/ml) ibonise ukuvimbela okusebenzayo ngokumelene ne-alpha-amylase.Okukhishiwe kuphinde kwahlolwa ukuvinjelwa kwe-alpha-glucosidase, futhi awukho umsebenzi ophawuliwe otholakele.Ukukhishwa kwe-Methanol kwe-C. rupestris kubonise umsebenzi ophawulekayo wokukhipha ama-radical mahhala (IC50 - 666.3 μg/ml), kulandele i-H2O2 (34%) ne-nitric oxide (49%).Ngaphezu kwalokho, iphrofayili yamakhemikhali eyenziwe yi-GC-MS yembula ukuba khona kwezinhlanganisela ezinkulu ze-bioactive.I-Phenol, 2,4-bis (1,1-dimethylethyl) kanye ne-z, z-6,28-heptatriactontadien-2-one itholakale kakhulu ekukhishweni kwe-methanol ye-C. rupestris kanye ne-chloroform ekhishwe ku-C. aerea.Imiphumela yethu ibonisa ukuthi i-algae ekhethiwe ibonisa ukuvinjelwa kwe-alpha-amylase okuphawulekayo nomsebenzi we-antioxidant.Ngakho-ke, ukubonakaliswa kwama-compounds asebenzayo kanye nokuhlolwa kwayo kwe-vivo kuzophawuleka.Kukhethwe ulwelwe olune oluluhlaza ukuze kuhlolwe i-alpha-amylase, i-alpha-glucosidase inhibitory, kanye nomsebenzi we-antioxidant ku-vitro C. aerea ne-Chlorodesmis kubonise ukuvimbela okuphawulekayo ngokumelene ne-alpha-amylase, futhi I-C. rupestris ibonise umsebenzi ophawulekayo wokuqothula ama-radical wamahhalaAwukho umsebenzi ophawuliwe otholakele ngokumelene nokuhlaziywa kwe-alpha-glucosidaseGC-MS kwezingcaphuno ezisebenzayo kuveza ukuba khona kwezinhlanganisela ezinkulu ezinikeza ukuqonda ngomsebenzi we-antidiabetic kanye ne-antioxidant walezi zingwenya.Izifinyezo ezisetshenzisiwe: DPPH: 2,2-diphenyl-1-picrylhydrazyl, BHT: Butylated hydroxytoluene, GC-MS: Gas chromatography-mass spectrometry.
