I-Biosynthesis ye-proteoglycans kanye ne-glycosaminoglycans ebukhoneni be-p-nitrophenyl-xyloside yacwaningwa kusetshenziswa uhlelo oluyisisekelo lwe-granulosa cell cell culture yegundane.Ukwengezwa kwe-p-nitrophenyl-xyloside esimisweni se-cellculture medium kubangele ukwanda okungama-700% kwe-[35S]sulfate incorporation (ED50 at 0.03 mM) kuma-macromolecules, ahlanganisa amaketanga e-chondroitin sulfate amahhala aqalwe ku-xyloside nama-proteoglycans omdabu.Amaketanga e-chondroitin sulfate amahhala aqalwa ku-xyloside acishe akhishwa phakathi nendawo.Usayizi wamangqamuzana wamaketanga e-chondroitin sulfate wehla ukusuka ku-40,000 kuya ku-21,000 njengoba ingqikithi ye- [35S] sulfate incorporation yathuthukiswa, okuphakamisa ukuthi ukuhlanganiswa okuthuthukisiwe kwe-chondroitin sulfate kwaphazamisa indlela evamile yokuqedwa kweketanga le-glycosaminoglycan.I-Biosynthesis ye-heparan sulfate proteoglycans yehliswe cishe ngo-50%, cishe ngenxa yokuncintisana ezingeni lama-precursors e-UDP-ushukela.[35S]I-sulfate incorporation yavalwa ngokungezwa kwe-cycloheximide ngesigamu sokuqala sesikhathi cishe samahora ama-2 lapho kukhona i-xyloside, kuyilapho ingekho i-xyloside cishe imizuzu engama-20.Umehluko cishe ubonisa izinga lenzuzo lomthamo wokuhlanganisa we-glycosaminoglycan usuwonke.Izinga lenzuzo lomthamo wokuhlanganisa we-glycosaminoglycan elibonwe kumaseli e-granulosa wesibeletho lalimfushane kakhulu kunalelo elibonwa kuma-chondrocyte, okubonisa ukubusa okuhlobene komsebenzi we-proteoglycan biosynthetic emsebenzini wawo wonke we-metabolic wamaseli.
