4-Nitrophenyl phosphate disodium usawoti 6-hydrate CAS:333338-18-4 okumhlophe kuya kumpushana ophuzi ophaphathekile
| Inombolo Yekhathalogi | XD90511 |
| Igama Lomkhiqizo | 4-Nitrophenyl phosphate disodium usawoti 6-hydrate |
| I-CAS | 333338-18-4 |
| I-Molecular Formula | I-C6H16NNa2O12P |
| Isisindo samangqamuzana | 371.142 |
| Imininingwane Yesitoreji | I-ambient |
| Ikhodi Yentela Evumelanisiwe | 29199000 |
Ukucaciswa Komkhiqizo
| Ukubukeka | okumhlophe ukuya kokuphuzi okuphaphathekile |
| Isivivinyo | 99% |
Ngaphezulu kwama-70% ama-glioma ebanga eliphansi athwala i-heterozygous R132H eguquguqukayo ku-gene coding ye-isocitrate dehydrogenase 1 (IDH1).Lokhu kunikeza i-enzyme ikhono lenoveli lokuguqula i-α-ketoglutarate ibe yi-2-hydroxyglutarate, ekugcineni kuholele ku-tumorigenesis.Umthombo omkhulu wokukhiqizwa kwe-2-hydroxyglutarate yi-glutamine, okuthi, kumdlavuza, futhi iwumthombo we-tricarboxylic acid cycle (TCA) anaplerosis.Omunye umthombo we-anaplerosis i-pyruvate flux nge-pyruvate carboxylase (PC), okuyindlela evamile kuma-astrocyte avamile.Umgomo walolu cwaningo kwakuwukuthola ukuthi i-PC isebenza njengomthombo we-TCA anaplerosis kumaseli aguquguqukayo e-IDH1 lapho i-glutamine isetshenziselwa ukukhiqizwa kwe-2-hydroxyglutarate.Ama-astrocyte avamile angafi aklanyelwe ukuveza i-heterozygous mutant IDH1 noma i-IDH1 yohlobo lwasendle yaphenywa.Ukugeleza kwe-pyruvate nge-PC nange-pyruvate dehydrogenase (PDH) kwanqunywa ngokusebenzisa i-spectroscopy ye-magnetic resonance ukuze kuphenywe ukulebula kwe-¹³C-led led ye-C-labe engu-¹³C]etholakala ku-glucose.Izivivinyo zomsebenzi, i-RT-PCR kanye ne-Western blotting kwasetshenziswa ukuze kuphenywe isisho nomsebenzi wama-enzyme afanelekile.Idatha yeCancer Genome Atlas (TCGA) yahlaziywa ukuze kuhlolwe ukuvezwa kwama-enzyme kumasampula e-glioma yabantu.Uma kuqhathaniswa namaseli ohlobo lwasendle, amaseli e-IDH1 aguquguqukayo andise kakhulu ukugeleza kwe-fractional nge-PC.Lokhu kuhlotshaniswa nokwanda okukhulu komsebenzi we-PC kanye nenkulumo.Ngasikhathi sinye, umsebenzi we-PDH wehle kakhulu, okungenzeka ukuthi uxhunywe ngokukhuphuka kakhulu kokuvimbela i-PDH phosphorylation nge-PDH kinase 3. Ngokuvumelana nokubhekwa kumaseli, ukuhlaziywa kwedatha ye-TCGA kubonise ukwanda okukhulu kwenkulumo ye-PC kumasampuli e-glioma e-IDH eveza i-IDH eshintshile uma kuqhathaniswa nesendle- thayipha i-IDH.Okutholakele kwethu kusikisela ukuthi izinguquko ku-PC kanye ne-PDH zingase zibe yingxenye ebalulekile yokujwayela kweselula ekuguquleni i-IDH1 futhi ingase isebenze njengezinhloso zokwelashwa ezingaba khona.
