Imikhiqizo

Ubufakazi bamuva nje busekela indima evelayo ye-β-nicotinamide adenine dinucleotide (β-NAD(+) ) njengenoveli neurotransmitter kanye neuromodulator ohlelweni lwezinzwa lwe-peripheral -β-NAD(+) ikhishwa kumalungiselelo emisipha ebushelelezi nezinzwa ze-adrenal chromaffin. ngendlela eyisici se-neurotransmitter.Okwamanje akukacaci ukuthi lokhu kuyiqiniso yini ku-CNS.Sisebenzisa i-superfusion assay yegumbi elincane kanye namasu okuzwela okuphezulu kwe-high-pressure liquid chromatography, sibonisa ukuthi ukukhuthazwa kwe-K(+) okuphezulu kwe-rat forebrain synaptosomes kuvusa ukuchichima kwe-β-NAD(+) , adenosine 5'-triphosphate, kanye i-metabolites i-adenosine 5'-diphosphate (ADP), i-adenosine 5'-monophosphate, i-adenosine, i-ADP-ribose (ADPR) kanye ne-cyclic ADPR.Ukuchichima okuphezulu kwe-K(+) -okukhishwe kwe-β-NAD(+) kuncishiswa ukuqhekeka kwe-SNAP-25 nge-botulinum neurotoxin A, ngokuvimbela iziteshi ze-N-type ezincike ku-voltage Ca(2+) ezine-ω-conotoxin GVIA , nangokuvinjwa kwe-proton gradient yama-synaptic vesicles ane-bafilo mycin A1, okuphakamisa ukuthi i-β-NAD(+) cishe ikhishwa nge-vesicle exocytosis.Ukuhlaziywa kwaseNtshonalanga kubonisa ukuthi i-CD38, iphrotheni esebenza ngezinto eziningi egaya i-β-NAD(+) , ikhona kulwelwesi lwe-synaptosomal naku-cytosol.Ama-synaptosomes angashintshile alulaza i-β-NAD(+) .I-1,N (6) -etheno-NAD, i-analog ye-fluorescent ye-β-NAD(+) , ithathwa ama-synaptosomes futhi lokhu kuthathwa kuncishiswa i-β-NAD(+) eyiqiniso, kodwa hhayi i-connexin 43 inhibitor Gap 27. Kuma-neurons e-cortical ukusetshenziswa kwasendaweni kwe-β-NAD(+) kubangela ukuguquguquka okusheshayo kwe-Ca(2+), cishe ngenxa yokuthutheleka kwe-Ca(2+) engaphandle.Ngakho-ke, ama-synaptosomes obuchopho begundane angakhulula, alulaze futhi athathe ngenkuthalo i-β-NAD(+) , futhi i-β-NAD(+) ingashukumisa ama-postsynaptic neurons, yonke imibandela edingekayo ukuze into ithathwe njenge-neurotransmitter ikhandidethi ebuchosheni.