Imikhiqizo

Ama-reagents e-Trinder amasha aphuma ngokuphuma emanzini ancibilika kakhulu asetshenziswa kakhulu ekuhlolweni kokuxilonga kanye nokuhlolwa kwe-biochemical.Kunezinzuzo ezimbalwa ngaphezu kwama-reagents ajwayelekile e-chromogenic ekunqumeni kombala womsebenzi we-hydrogen peroxide.Ama-reagents e-Trinder amasha azinzile ngokwanele ukuze asetshenziswe kukho kokubili isixazululo namasistimu okuhlola amapayipi.Ebukhoneni be-hydrogen peroxide ne-peroxidase, i-reagent yenoveli ye-Trinder yaboniswa ukuthi isabela nge-4- aminoantipyrine (4-AA) noma i-3- methylbenzothiazole sulfonehydrazone (MBTH) phakathi nokusabela kokuhlangana kwe-oxidative.Yakha odayi be-violet noma abaluhlaza okwesibhakabhaka abazinzile.I-molar absorbance yedayi ehlanganiswe ne-MBTH yayiphakeme izikhathi ezingu-1.5- 2 kunaleyo yedayi ehlanganiswe ne-4-AA;noma kunjalo, isisombululo se-4-AA sasizinzile kunesixazululo se-MBTH.I-substrate i-enzymatic oxidized nge-oxidase yayo ukuze ikhiqize i-hydrogen peroxide.Ukugxila kwe-hydrogen peroxide kuhambisana nokuhlushwa kwe-substrate.Ngakho-ke, inani le-substrate linganqunywa ukuthuthukiswa kombala we-oxidative coupling reaction.IGlucose, utshwala, i-acyl-CoA kanye ne-cholesterol kungasetshenziswa ukuthola lawo ma-substrates ahlanganiswe nenoveli ye-Trinder's reagent kanye ne-4-AA.Kukhona ama-reagents amasha ayi-10 e-Trinder atholakalayo.Phakathi kwama-reagents amasha e-Trinder, i-TOOS isetshenziswa kakhulu.Kodwa-ke, kwi-substrate ethile, ukuhlola amakilasi ahlukene ama-reagents anoveli e-Trinder kuyadingeka ukuze kuthuthukiswe isistimu yokuthola efanele.