I-Reagent entsha ye-Trinder iwukuphuma kwe-aniline encibilika kakhulu emanzini esetshenziswa kabanzi ekuhlolweni kokuxilonga nokuhlola amakhemikhali e-biochemical.Inezinzuzo ezimbalwa ngaphezu kwama-reagents ajwayelekile e-chromogenic ekunqumeni kombala womsebenzi we-hydrogen peroxide.I-Reagent entsha ye-Trinder izinze ngokwanele kukho kokubili Ingasetshenziswa ekuxazululeni noma ohlelweni lokuhlola amapayipi.Lapho kukhona i-hydrogen peroxide kanye ne-peroxidase, i-reagent entsha ye-Trinder'si ingasetshenziswa ngokuhambisana ne-4-aminoantipyrine (4-AA) noma i-3 methyl Ngesikhathi sokuhlangana kwe-oxidative ye-benzothiazole sulfonehydrazone (MBTH), odayi abazinzile kakhulu abansomi noma abaluhlaza okwesibhakabhaka. kwakheka.I-molar absorbance of the dyes ehambisana ne-MBTH iphakeme izikhathi ezingu-1.5-2 kunezodayi ezihambisana ne-4-AA;noma kunjalo, i-4-AA Isixazululo sizinzile kunesixazululo se-MBTH.I-substrate i-enzymatic oxidized yi-oxidase yayo ukuze ikhiqize i-hydrogen peroxide.Ukuhlushwa kwale hydrogen peroxide kuhambisana nokuhlushwa kwe-substrate.Ngakho-ke, inani le-substrate linganqunywa ukuthuthukiswa kombala we-oxidative coupling reaction.IGlucose , utshwala, i-acyl-CoA, kanye ne-cholesterol kungasetshenziswa ukuthola lawo ma-substrates ahlanganiswe nama-reagents e-Trinder anoveli kanye ne-4-AA.Kukhona ama-reagents amasha ayi-10 e-Trinder.Phakathi kwama-reagents amasha e-Trinder, i-TOOS isetshenziswa kakhulu.Kodwa-ke, ku-substrate ethile, ukuhlola izinhlobo ezahlukene zamanoveli ama-Trinder's reagents kuyadingeka ukuze kuthuthukiswe isistimu yokuthola efanele.
