Imikhiqizo

Amafilimu e-Biofilm akhiwe ngamaseli ebhaktheriya ambozwe ku-matrix ye-polymeric eyenziwe ngokwayo, engaphandle kweselula.I-Poly-beta(1,6)-N-acetyl-d-glucosamine (PNAG) iyingxenye enkulu ye-biofilm matrix kumabhaktheriya ahlukahlukene we-phylogenetically.Kulolu cwaningo siphenye izici ezibonakalayo namakhemikhali ze-matrix ye-PNAG kuma-biofilms akhiqizwa ku-vitro yi-gram-negative porcine pathogen yokuphefumula i-Actinobacillus pleuropneumoniae kanye ne-gram-positive device-associated pathogen i-Staphylococcus epidermidis.Umthelela we-PNAG ekugelezeni koketshezi ngobuningi wanqunywa ngokulinganisa izinga lokuguquguquka koketshezi ngama-biofilms akhuliswe kumishini yesihlungi se-centrifugal.Izinga lokuguquguquka koketshezi laliphezulu kakhulu kuma-biofilms akhuliswe phambi kwe-enzyme eyonakalisa i-PNAG i-dispersin B kunakuma-biofilms akhuliswe ngaphandle kwe-enzyme, okubonisa ukuthi i-PNAG yehlisa ukugeleza koketshezi ngobuningi.I-PNAG iphinde yavimba ukuthuthwa kwe-quaternary ammonium compound cetylpyridinium chloride (CPC) ngama-biofilms.Ukubophezela kwe-CPC kuma-biofilms kuphinde kwavimbela ukuguquguquka koketshezi futhi kwavinjwa ukuthuthwa kwedayi ye-azo i-Allura ebomvu.I-Bioactive CPC yakhishwa ngempumelelo kuma-biofilms ngokwelashwa nge-1 M sodium chloride.Sekuhlanganiswe ndawonye, ​​lokhu okutholakele kusikisela ukuthi i-CPC iphendulana ngqo ne-PNAG matrix futhi iguqule izakhiwo zayo ezingokomzimba namakhemikhali.Imiphumela yethu ibonisa ukuthi i-PNAG idlala indima ebalulekile ekulawuleni isimo somzimba wama-biofilms futhi ingase ibe nomthelela ezinqubweni ezengeziwe ezihambisana ne-biofilm ezifana nokumelana ne-biocide.