Imikhiqizo

Enye indlela yokwelapha ebalulekile ekwelapheni uhlobo 2 sikashukela i-mellitus ukusetshenziswa kwama-ejenti anganciphisa i-postprandial hyperglycemia ngokuvimbela ama-enzyme wokugaya ama-carbohydrate.Ucwaningo lwamanje luphenye imiphumela yokukhishwa okuqondiswa yi-bioassay kanye nezingxenyana zesithelo esomisiwe se-pericarp ye-Phaleria macrocarpa, isitshalo sendabuko esilwa nesifo sikashukela, ku-α-glucosidase ne-α-amylase, emzamweni wokuqonda indlela yabo yokulwa nesifo sikashukela, njengoba kanye nesenzo sabo sokuncisha amandla okungenzeka ekunyukeni kweglucose yangemva kokudla. Ukukhishwa kwe-Methanol (ME), okutholwe ngokukhipha isincibilikisi okulandelanayo, ingxenye yayo esebenza kahle kakhulu ye-liquid-liquid n-butanol (NBF) kanye ne-flash column chromatographic sub-fraction (SFI), kwahlolwa. ye-in vitro α-glucosidase (yeast) kanye ne-α-amylase (ingulube) yokuvimbela umsebenzi.Ngaphezu kwalokho, ukuhlolwa kwe-vivo kwe-vivo kwenziwa kumagundane e-diabetesic streptozotocin-induced Diabetic (SDRs) kusetshenziswa ukuhlolwa kokubekezelela ushukela, i-sucrose kanye nesitashi. 75%) kanye ne-α-amylase (87%) ku-vitro (IC50 = 2.40 ± 0.23 μg/ml kanye no-58.50 ± 0.13 μg/ml, ngokulandelana) ngendlela encike kumthamo;umphumela otholakala ucishe ube ngu-20% ngaphezu kwe-acarbose (55%), i-α-glucosidase inhibitor evamile (IC50 = 3.45 ± 0.19 μg/ml).I-ME ne-SFI iphinde yavimbela i-α-glucosidase (IC50 = 7.50 ± 0.15 μg/ml kanye no-11.45 ± 0.28 μg/ml) kanye ne-α-amylase (IC50 = 43.90 ± 0.19 μg/ml) ± 0.19 μg/ml/ml ± 0.9ml no-6 to μg. ngezinga elincane.Ezifundweni ze-vivo ezinamagundane esifo sikashukela, i-NBF ne-SFI yehlise ngempumelelo i-peak blood glucose (PBG) ngo-15.08% no-6.46%, kanye nendawo engaphansi kwe-tolerance curve (AUC) ngo-14.23% no-12.46%, ngokulandelana, ngemva kwenselelo yomlomo ye-sucrose. (P <0.05);ngalokho kuqinisekisa isenzo esibonwayo se-in vitro.Le miphumela yokunciphisa ku-PBG ne-AUC yaboniswa nasekuhlolweni kokubekezelela ushukela kanye nesitashi, kodwa ngezinga eliphansi.Lokhu okutholakele kuveza ukuthi i-P. macrocarpa inganciphisa i-hyperglycemia kuzo zombili izimo ze-in vitro kanye ne-vivo ngokuvimbela ngamandla ama-enzyme e-carbohydrate hydrolysing, ikwenze isitshalo esisebenzayo sokuthola izinhlanganisela zemvelo zokuphatha uhlobo 2 sikashukela i-mellitus.