Imikhiqizo

Amaqembu amathathu ezinkawu zesilisa ezikhulile ze-cynomolgus (i-Macaca fascicularis) avezwe ku-200 micrograms/m3 ammonium hexachloroplatinate [(NH4)2PtCl6], 200 micrograms (NH4)2PtCl6 kanye ne-1 ppm ozone (O3), noma ku-O31 kuphela.Izilwane zavezwa ngokuhogela umoya amahora ayisi-6 ngosuku, izinsuku ezi-5 ngeviki amaviki ayi-12.Idizayini yokuhlola yayihlanganisa ukuhlolwa kwangaphambili kwe-methacholine kanye nokuhlolwa kwenselele ye-bronchoprovocation ye-Na2PtCl6, ukuhlolwa kwesikhumba somkhawulo we-Na2PtCl6, kanye ne-sera yokuhlaziya amasosha omzimba.Emasontweni amabili ngemva kokuchayeka kwamasonto angu-12, lezi zinkomba ziye zabuyekezwa.Umsebenzi wamaphaphu wokuqala awuzange uthinteke kakhulu yimithi yokuchayeka;kodwa-ke, inhlanganisela yokuchayeka ku-O3 kanye ne-(NH4)2PtCl6 inciphise kakhulu ukugcwala kweplatinamu (Pt) usawoti kanye ne-methacholine edingekayo ukuze kwandiswe ukumelana nokugeleza kwamaphaphu okumaphakathi (RL) 200% (EC200 RL).Ukuchayeka kwe-ozone noma i-Pt kukodwa akuzange kube nomthelela obalulekile kulawa mapharamitha.Amanani ePlatinum kanye ne-methacholine EC200 RL aye ahlotshaniswa kakhulu kuwo womabili amaqembu e-Pt-eveziwe ngemva kokuchayeka.Le datha ibonise ukuthi ukuchayeka kwe-O3 ne-Pt okuhlanganisiwe kwenyuke kakhulu okuqondile (Pt) nokungaqondile (i-methacholine) kwe-bronchial hyperreactivity kaningi kunokuchayeka ku-O3 noma kusawoti we-Pt kuphela.Ukuchayeka okuhlanganisiwe kwe-O3 kanye ne-Pt nakho kukhulisa kakhulu izehlakalo zokuhlolwa kwe-Pt isikhumba esihle uma kuqhathaniswa namanye amaqembu okuchayeka.Ngokufanayo nolwazi lomuntu, ukuhlolwa kwe-radioallergosorbent (RAST) kwamasosha omzimba aqondene ne-Pt kwakungazweli njengokuhlolwa kwesikhumba okuqondile ekuhlonzeni abantu abaguliswa yizifo.