Imikhiqizo

I-APS-5 iyi-substrate ye-chemiluminescent esekelwe ku-9,10-dihydroacridine, esetshenziselwa ikakhulukazi ukutholwa kwe-ELISA kwama-compounds nge-alkaline phosphatase AP conjugates;I-9,10-dihydroacridine esakhiweni se-APS-5 Isakhiwo se-hydroacridine singakhipha ukukhanya ngokuqhubekayo, ngokuzinza nangempumelelo ngaphansi kwesenzo se-AP, futhi i-APS-5 ifaneleka kakhulu ekuhlolweni kwesisombululo somsebenzi we-phosphatase kanye ne-phosphatase enzyme-linked immunoassay.

Njenge-luminescent probe, isetshenziswa ekutadisheni ama-gene chips.Ukusabela kubhalwe nge-acridan (9,10-dihydroacridine) njenge-substrate ne-alkaline phosphatase, ekhiqiza i-chemiluminescence ephezulu eqinile.Inikeza ukuzwela okuphakeme kanye nokusebenziseka kalula kwe-alkaline phosphatase conjugation ngesikhathi sokutholwa kwe-chemiluminescent.I-alkaline phosphatase itholwa ngaphansi kuka-10-19 mol, iphakama ngokushesha ukuze kuncishiswe isikhathi sokubona nokwandisa ukuphuma, futhi ukuthambeka kwejika lokulinganiswa komugqa kuhlelwa nge-logarithmic elingana no-1.0.Inani elilodwa noma ngaphezulu kwe-enzyme ikhiqiza inani elilodwa noma ngaphezulu kwe-luminescence, i-luminescence eqhubekayo-engafuni kakhulu ngesikhathi sokuhlolwa.Ukuqina kwe-Luminescence kungafundwa ejikeni lokulinganisa lomugqa elikhiqiziwe noma nini, futhi imiphumela yokuhlaziya ayizwani nezinga lokushisa ebangeni elingu-22°C - 35°C, linciphisa ukunemba okudingekayo ukuze kulawulwe izinga lokushisa.

inzuzo:

1 Ukuzwela okuphezulu - izimpawu zamaprotheni ngaphansi kwe-5pg zingatholwa;

2 Amandla aphezulu okukhanya - isiqongo esikhanyayo singafinyelelwa ngesikhathi esifushane;

3 I-luminescence eqhubekayo futhi ezinzile - inqubo yayo ye-luminescence ayithinteki izinga lokushisa ngaphakathi kwama-25-35 ℃, futhi ayidingi ukulawulwa kwezinga lokushisa.

Ukuqhathaniswa kohlelo lokusebenza kwama-substrates e-luminescent avame ukusetshenziswa APS-5 (193884-53-6) kanye ne-AMPPD (122341-56-4)

Kokubili i-APS-5 (193884-53-6) kanye ne-AMPPD (122341-56-4) zingama-chemiluminescent substrates e-alkaline phosphatase, futhi zombili zibizwa ngokuthi ama-ultra-high sensitivity reagents, ngakho lawa ma-reagents amabili atholakala ekutholeni ukuthi Yikuphi okungcono futhi yikuphi okubi kakhulu?Ungakhetha kanjani uma usebenzisa?

Ngakolunye uhlangothi, lapho i-APS-5 isetshenziswa njengengxenye yokutholwa kwe-alkaline phosphatase, ukuzwela kokutholwa kungafinyelela ekugxilweni kwe-molar eyi-10-19, isikhathi sokuthuthukiswa kombala singafinyelela inani eliphakeme kakhulu ngemizuzwana eyi-10, futhi i-fluorescence ihlala isikhathi eside. isikhathi.Kulezi zici, i-APS 5 kungathiwa inezakhiwo ezinhle kakhulu.Kodwa-ke, i-APS-5 ikhawulelwe kuzinga lokushisa lohlelo lokusebenza, futhi izinga lokushisa layo lokunikeza umbala kungcono libe ngaphansi kuka-20'C.Ngakolunye uhlangothi, uma i-AMPPD isetshenziswa njengengxenye etholwayo ye-alkaline phosphatase, ukuzwela kokutholwa kubuye kubizwe ngokuthi ukuzwela okuphezulu kakhulu, kodwa akukwazi ukufinyelela izinga le-APS 5. Isikhathi sokuthuthukisa umbala sifinyelela phezulu kakhulu cishe amasekhondi angu-30. , kanye nobude be-fluorescence bude.I-AMPPD yinhle kakhulu kulezi zakhiwo ezingenhla, kodwa ngokuyisisekelo ingaphansi kwe-APS5, kodwa izinga lokushisa layo lokunikeza umbala aliqinile njenge-APS5, futhi izinga lokushisa lokulondoloza elingu-2--8C lilula kune-APS-5.Kafushane, nakuba i-APS-5 idlula i-AMPPD ngokunemba kokutholwa, ilahlekelwa ukuzinza.Indlela yokuyikhetha incike ezidingweni ezithile zokutholwa.

1. Ukwethulwa kwe-APS-5 (193884-53-6):

Igama lesiNgisi: APS-5

Inombolo ye-CAS: 193884-53-6

Ifomula yemolekyuli: C21H15ClNO4PS Na2

Isisindo se-molecule: 489.819

Ukubukeka: impushana eqinile emhlophe emhlophe

Okuqukethwe: ≥98%

Imininingwane: 1g, 5g, 10g

Izimo zokugcina: -20 ℃ ukulondolozwa

(4-Chlorophenylmercapto)(10-methyl-9,10-dihydroacridine methylene) usawoti we-phosphate disodium, obizwa ngokuthi i-APS-5, i-reagent ye-chemiluminescent futhi isetshenziswa njenge-substrate ye-chemiluminescent.Inikeza ukuzwela okuphakeme kanye nokusebenziseka kalula kwe-alkaline phosphatase conjugation ngesikhathi sokutholwa kwe-chemiluminescent.Ukusabela kubhalwe nge-acridan (9,10-dihydroacridine) njenge-substrate ne-alkaline phosphatase, ekhiqiza i-chemiluminescence ephezulu eqinile.I-APS-5 iyi-reagent efanelekile yokunqunywa komsebenzi we-phosphatase ngokuhlolwa kwesixazululo kanye nokuhlolwa kwe-immunoassay okuxhumene ne-enzyme ye-phosphatase.Njenge-luminescent substrate, i-APS-5 inokuzwela kokutholwa okuphezulu kanye nesikhathi esifushane sokuphendula.

2. Ukwethulwa okuningiliziwe kwe-AMPPD (122341-56-4)

Igama lesiNgisi: AMPPD

Inombolo ye-CAS: 122341-56-4

Ifomula yemolekyuli: C18H23O7P

Isisindo se-molecule: 382.344781

Ukubukeka: impushana emhlophe

Ubumsulwa: ≥97%

Imininingwane: 1g, 5g, 10g

Izimo zokugcina: -20 ℃ ukulondolozwa

Izinyathelo zokuqapha: Kumele yome ukuze ugweme ukuqedwa njalo kanye ne-lyophilization