Imikhiqizo

I-Bicine iyibhafa ye-zwitterionic amino acid, isebenza kububanzi be-pH 7.6-9.0 (pKa ka-8.26 ku-25°C).Isigcinalwazi esinconyiwe somsebenzi wamakhemikhali e-biochemical okushisa aphansi.I-Bicine isetshenziselwa ukulungiswa kwesisombululo se-substrate esizinzile sokunqunywa kwe-serum guanase.Ukusetshenziswa kwe-bicine kungqimba oluncane lwe-ion exchange chromatography indlela yokuxazulula amaprotheni kushicilelwe.I-Bicine isetshenziswe ku-peptide kanye ne-protein crystallization.Ucwaningo lwe-kinetic lwenkimbinkimbi ye-quaternary transition-state analogue ye-creatine kinase isebenzise i-bicine kubhafa yokusabela.Uhlelo lwe-multiphasic buffer lwe-SDS-PAGE lwamaprotheni nama-peptide ahlanganisa i-bicine luchaziwe.

Izindonga ze-alveolar eyodwa ezingaphansi kwezifundo zokuqina kobude ku-saline kanye ne-Bicine (0.2 M) zibe nokubola okuqhubekayo ku-tissue tension (TTD).Sihlole umthelela wezisombululo ezihlukene kule TTD futhi sabheka izinguquko ezihambisanayo ku-ultrastructure.I-parenchyma yamaphaphu yahlakazwa yaba ezindongeni ze-alveolar eyodwa (30 X 30 X 150 microns) kusawoti ovikelwe yi-phosphate (0.15 M).Idluliselwe kubhavu odonsa isikhathi eside, izicubu zacwiliswa ku-Bicine, saline, isixazululo se-Hank esiqinile, 0.25% i-Alcian eluhlaza okwesibhakabhaka ku-saline, noma isixazululo se-sodium dodecyl sulfate, ngezikhathi eziguquguqukayo.Ihamba ngebhayisikili ngesandiso esinikeziwe esinamandla amakhulu akalwa ngokuhamba kwesikhathi, lezi zicubu ezifanayo zalungiswa ku-glutaraldehyde/tannic acid ebhafa futhi zacutshungulwa ukuze zithole i-electron microscopy.Izindonga ze-alveolar eyodwa ezicwiliswe ku-saline noma i-Bicine zibonise i-TTD eqhubekayo.Ama-vacuoles noma izikhala zivele ku-interstitium okwathi ngokuphazamiseka kwamaselula kwaqhubekela phambili ne-TTD.Ibonwe phakathi namahora angu-0.3, izinguquko zazithuthuke kakhulu ku-0.6 h.Ku-sodium dodecyl sulfate (70 mM), nokho, yayingekho i-TTD futhi ngokwesakhiwo yayingekho i-interstitium, kwakusele ulwelwesi olungaphansi kanye namaprotheni anemicu.Kusixazululo sika-Hank esiqinile noma u-0.25% we-Alcian eluhlaza okwesibhakabhaka i-matrix ye-interstitial, i-cell morphology kanye nokungezwani kwezicubu kulondolozwe kahle ihora elingu-1.Lolu cwaningo luphakamisa ukuthi ukucwiliswa kwe-matrix ye-interstitial kwenzeka ku-saline noma ku-Bicine, futhi i-matrix eqinile ibalulekile ukuze kulondolozwe ukungezwani kwezicubu.