![I-Bis[2-Hydroxyethyl] imino Tris-(Hydroxymethyl)-methane Cas: 6976-37-0 99% Isithombe Esifakiwe](http://cdn.globalso.com/xdbiochems/6976-37-0.jpg)
I-Bis[2-Hydroxyethyl] imino Tris-(Hydroxymethyl)-methane Cas: 6976-37-0 99%
| Inombolo Yekhathalogi | I-XD90111 |
| Igama Lomkhiqizo | I-BIS-TRIS (Bis(2-hydroxyethyl)amino-tris(hydroxymethyl)methane) |
| I-CAS | 6976-37-0 |
| I-Molecular Formula | I-C8H20NO5 |
| Isisindo samangqamuzana | 210.2476 |
| Imininingwane Yesitoreji | I-ambient |
| Ikhodi Yentela Evumelanisiwe | 2922190090 |
Ukucaciswa Komkhiqizo
| Izinsimbi ezinzima | <10ppm |
| Izinsalela ekushiseni | <0.1% |
| I-Pka | 6.3-6.7 |
| Ukubukeka | I-Crystalline Powder Emhlophe |
| I-Solubility (1M ku-H20) | Kucacile Futhi Kuphelele |
| i-pH (1M ku-H2O) | 9.0-11.0 |
| Umswakama (LOD) | 1.0% ubuningi. |
| I-Assay (I-Titration on dry basis) | 99.0% iminithi. |
I-buffer, esetshenziselwa ukutholwa kwe-creatine-creatinase;futhi isetshenziselwa ukuvikela okusebenzayo kwe-hemoglobin ekomisweni kweqhwa;catholyte, esetshenziswa IEF/2-D ijeli electrophoresis.
I-Glial cell-derived neurotrophic factor (GDNF) iyisivimbeli esinamandla sokusetshenziswa kwe-ethanol nokuphinda siphinde, futhi amagundane e-GDNF heterozygous knockout abonisa ukuzwela komvuzo ku-ethanol futhi adle i-ethanol eningi ngemva kwesikhathi sokuzithiba kunama-littermate awo ohlobo lwasendle.Lapha, sihlole ukuthi i-ethanol iyashintsha yini inkulumo ye-GDNF endaweni ye-ventral tegmental (VTA; indawo yokusebenza ye-GDNF) kanye/noma i-nucleus accumbens (NAc; umthombo oyinhloko we-GDNF), futhi uma kunjalo, nquma indima yesici sokukhula esingapheli. ekulawuleni ukusetshenziswa kwe-ethanol.Ukuphathwa okuhlelekile kwe-ethanol kukhuphule ukubonakaliswa kwe-GDNF namazinga amaprotheni ku-VTA, kodwa hhayi i-NAc.Ukwengeza, amazinga e-GDNF aphakanyisiwe ngemva kweseshini yokuphuza i-ethanol kumagundane adle i-ethanol ekufinyeleleni okuphakathi kwenqubo yokukhetha amabhodlela amabili eviki le-1, kodwa hhayi amaviki angu-7.Ukwehliswa okulandela amaviki angu-7 okuphuza ngokweqile kwe-ethanol kunciphisa amazinga e-GDNF, kuyilapho isikhathi esifushane sokuphuza i-ethanol ngemuva kokwehliswa kwenkulumo ye-GDNF elawulwayo.Okubalulekile, ukudilizwa kwe-GDNF ngaphakathi kwe-VTA kusetshenziswa i-adenovirus eveza i-hairpin emfushane i-RNA kusize ukwanda kokuphuza i-ethanol ngamagundane ane-ethanol-naïve, kodwa hhayi ngamagundane anomlando wokuphuza i-ethanol ngokweqile.Le miphumela iphakamisa ukuthi phakathi nokuhlangenwe nakho kokuqala kokuphuza i-ethanol, i-GDNF ku-VTA iyanda futhi ivikela ekuthuthukisweni kokuthatha i-ethanol ngokweqile.Kodwa-ke, impendulo yokuvikela yesici sokukhula ku-ethanol iyancipha ngemva kokuthatha isikhathi eside ngokweqile kwe-ethanol nokuhoxiswa, okuholela ekusetshenzisweni okuphikelelayo, ngokweqile kwe-ethanol.
