Imikhiqizo

I-MES Buffer Ithinta I-Arabidopsis Root Apex Zonation kanye Nokukhula Kwezimpande Ngokucindezela Isizukulwane Se-Superoxide ku-Root Apex

Ezitshalweni, ukukhula kwezimpande nezinwele zezimpande kulawulwa ukulawulwa okuhle kwamaselula kwe-pH kanye nezinhlobo ze-oxygen esebenzayo (ROS).I-MES, 2-(N-morpholino)ethanesulfonic acid njengesinye se-Good's buffers isetshenziswe kabanzi endaweni yokubhafa, futhi kucatshangwa ukuthi ifanele ukukhula kwezitshalo ngokugxila ku-0.1% (w/v) ngenxa yokuthi umthamo we-buffer I-MES esukela ku-pH 5.5-7.0 (ye-Arabidopsis, pH 5.8).Kodwa-ke, imibiko eminingi ibonise ukuthi, ngokwemvelo, izimpande zidinga amanani ahlukile we-pH endaweni ethile yezimpande ze-apex zones, okuyi-meristem, indawo yoshintsho, kanye nendawo yokwelula.Ngaphandle kweqiniso lokuthi izimpande zihlala zikhula kumidiya equkethe i-molecule ye-buffer, kuncane okwaziwayo ngomthelela we-MES ekukhuleni kwezimpande.Lapha, sihlole imiphumela yokugxila okuhlukile kwe-MES buffer sisebenzisa izimpande ezikhulayo ze-Arabidopsis thaliana.Imiphumela yethu ibonisa ukuthi u-1% we-MES uvimbele kakhulu ukukhula kwezimpande, inani lezinwele zezimpande nobude be-meristem, kuyilapho u-0.1% ekhuthaza ukukhula kwezimpande kanye nendawo ephakeme yezimpande (isifunda esisuka esihlokweni sezimpande siye endaweni yenguquko).Ngaphezu kwalokho, ukukhiqizwa kwe-superoxide ku-root apex kwanyamalala ku-1% we-MES.Le miphumela iphakamisa ukuthi i-MES iphazamisa i-root morphogenesis evamile ngokushintsha i-ROS homeostasis ku-root apex.