Imikhiqizo

I-Tricine, i-zwitterionic buffer reagent igama layo lisuselwa ku-Tris ne-glycine.Ukwakheka kwayo kufana ne-Tris, kodwa ukugxila kwayo okuphezulu kunomsebenzi ovimbelayo obuthakathaka kune-Tris.Enye yama-reagents ebhafa ye-Good, eyasungulwa ekuqaleni ukuhlinzeka ngohlelo lwe-buffer lokusabela kwe-chloroplast.Ububanzi bebhafa ye-pH obusebenzayo be-Tricine bungu-7.4-8.8, pKa=8.1 (25 °C), futhi ngokuvamile busetshenziswa njengesibaphi esisebenzayo kanye nokumisa kabusha ama-cell pellets.I-Tricine inezici zokushajwa okunegethivu okuphansi namandla aphezulu e-ionic, afaneleka kakhulu ukuhlukaniswa kwe-electrophoretic yamaphrotheni esisindo samangqamuzana aphansi angu-1~100 kDa.Esivivinyweni se-ATP esisekelwe kukhanyikhanyi luciferase, ukuqhathanisa amabhafa avamile ayi-10, i-Tricine (25 mM) ibonise umphumela wokuthola ongcono kakhulu.Ngaphezu kwalokho, i-Tricine futhi iyi-hydroxyl radical scavenger esebenzayo ekuhlolweni kokulimala kolwelwesi olubangelwa ama-radical.