Imikhiqizo

Isetshenziselwa ukwenza ibhafa ye-CAPS, isibhafa se-zwitterionic esiwusizo ebangeni le-pH 7.9-11.1.I-CAPS buffer isetshenziswa kakhulu ocwaningweni lwaseNtshonalanga nolwe-immunoblotting kanye nokulandelana kwamaprotheni kanye nokuhlonza.Isetshenziswa ekudluliselweni kwe-electro amaprotheni ku-PVDF (sc-3723) noma i-nitrocellulose membranes (sc-3718, sc-3724).I-pH ephezulu yale buffer ikwenza kube usizo ekudluliseni amaprotheni nge-pI> 8.5.kanye nokusebenza kabusha okuncane ngama-enzyme noma amaprotheni, imiphumela encane kasawoti.

Ku-capillary zone electrophoresis isivinini se-electrophoretic ye-ion siyehla njengoba ukugxila kwesixazululo se-electrolyte sangemuva kukhuphuka.Lokhu kubangelwa izinguquko ekuhambeni kwe-electrophoretic ye-ion (muep) kanye nezinguquko kumandla anetha ayithintayo, okungukuthi amandla enkundla kagesi asebenzayo (Eeff).Ukuhamba kwe-electrophoretic kwe-ion kuguqulwa ngezinguquko ku-viscosity ephelele yesisombululo se-electrolyte kanye nezinguquko kusayizi oxazululiwe we-ion.I-Eeff ishintshwa ngokuyinhloko izinguquko kubukhulu bomphumela we-asymmetry wokushaja kanye nomphumela we-electrophoretic, kokubili okubuyisela ukunyakaza kwama-ion.Kulolu cwaningo, indlela yomaka amathathu yasetshenziselwa ukutadisha umphumela wokuhlushwa kwe-electrolyte yangemuva (0.02-0.08M 3-[cyclohexylamino] -1-propanesulfonic acid ne-counter ion (Li, Na, K, ne-Cs) ku-Eeff. Kutholwe ukuthi ukugxiliswa kwe-electrolyte yangemuva kuyithinta ngokuphawulekayo i-Eeff nokuthi i-Eeff isondela ku-E njengoba ukugxiliswa kwe-electrolyte yangemuva kusondela ku-zero. I-counter ion ibe nomthelela omncane ku-Eeff: njengoba usayizi werediyasi enamanzi we-counter ion ukhula. , i-Eeff yehlile. Indlela yomaka amathathu ibonakale iphumelela ekunqumeni okunjalo.