Imikhiqizo

Umphumela we-acidosis, uphawu lwamakhemikhali ezinto eziphilayo ze-tumor microenvironment, ekuqhubekeleni phambili komdlavuza kanye ne-metastasis iyinkimbinkimbi.Kokubili imiphumela ekhuthazayo kanye ne-anti-tumorigenic ye-acidosis ibikiwe futhi imvelo ene-asidi iye yaxhashazwa ukulethwa kwezidakamizwa ezithile, ama-imaging agents, kanye nezakhi zofuzo ezakhiwe zibe izimila.Kulolu cwaningo siphenya ukusabalala nokunamathela okugxile kwamaseli e-melanoma e-B16F10 enziwe ngofuzo ukuze aveze ngokwedlulele i-pH-sensing G protein-coupled receptor GPR4.Ngokusebenzisa izivivinyo zokunamathiselwe kwiseli sithole ukuthi i-GPR4 overexpression ilibazise ukusakazeka kweseli futhi yashintsha ukwenziwa kwasendaweni kwendawo ye-dynamic focal adhesion complex, njengokwenziwa kwasendaweni kwe-phosphorylated focal adhesion kinase (FAK) kanye ne-paxillin, ku-acidic pH.Amaphrotheni e-G angaba khona kanye nezindlela zokusayina ezansi nomfula ezinesibopho sale miphumela nazo zaphenywa.Ngokusebenzisa i-Rho inhibitor CT04 (C3 transferase), i-Rho-associated kinase (ROCK) inhibitors Y27632 kanye ne-thiazovivin, i-myosin light chain kinase (MLCK) inhibitor staurosporine noma i-G12/13 inhibitory construct, ukusabalalisa kwamaseli kwabuyiselwa lapho ukwenziwa kusebenze kwezindlela ze-Gq ne-Gs kube nomthelela omncane noma kwangenawo nhlobo.Sekukonke imiphumela yethu ikhombisa ukuthi ngokusebenzisa indlela ye-G12/13/Rho yokusayina i-GPR4 imodela amandla okunamathela okugxilile futhi inciphise ukusabalala kwamaseli kanye nokugoqeka kwe-membrane.