Imikhiqizo

Izici zemvelo, njengokwakheka kwe-macronutrient yokudla, zingaba nomthelela omkhulu engcupheni yesifo sikashukela kanye nesifo se-metabolic.Ocwaningweni lwamanje sibonisa ukuthi into eyodwa, elula yokudla - i-leucine - ingaguqula ukumelana ne-insulin ngokusebenza ezicutshini eziningi nakumazinga amaningi e-metabolism.Amagundane abekwe ekudleni okujwayelekile noma okunamafutha aphezulu (HFD).I-leucine yezokudla yaphindwa kabili ngokungezwa emanzini okuphuza.I-mRNA, amaprotheni kanye namaphrofayili aphelele we-metabolomic ahlolwa ezicutshini ezinkulu ezizwela i-insulin neserum, futhi ahlotshaniswa nezinguquko ku-glucose homeostasis nokusayina kwe-insulin.Ngemuva kwamaviki angu-8 ku-HFD, amagundane aqala ukukhuluphala, isibindi esinamafutha, izinguquko ezivuthayo kuzicubu ze-adipose kanye nokumelana ne-insulin ezingeni le-IRS-1 phosphorylation, kanye nokuguqulwa kwephrofayili ye-metabolomic ye-amino acid metabolites, i-TCA cycle intermediates, i-glucose ne-cholesterol metabolites. , kanye nama-acids anamafutha esibindi, imisipha, amafutha kanye ne-serum.I-leucine yezokudla ephindaphindeka kabili ibuyise ukungalungi okuningi kwe-metabolite futhi yabangela ukuthuthuka okuphawulekayo ekubekezeleleni i-glucose kanye nokusayina kwe-insulin ngaphandle kokushintsha ukudla noma ukukhuluphala.Ukwenyuka kwe-leucine yezokudla kwahlotshaniswa nokuncipha kwe-hepatic steatosis kanye nokuncipha kokuvuvukala kwezicubu ze-adipose.Lezi zinguquko zenzeke naphezu kokwanda kwe-insulin-stimulated phosphorylation ye-p70S6 kinase ebonisa ukusebenza okuthuthukisiwe kwe-mTOR, into evame ukuhlotshaniswa nokungazweli kwe-insulin.Le datha ibonisa ukuthi izinguquko ezinesizotha endaweni eyodwa yezemvelo/izakhi zingashintsha izindlela eziningi ze-metabolic kanye nezimpawu futhi ziguqule i-HFD eyenziwe yi-metabolic syndrome ngokusebenza ezingeni lesistimu kumathishu amaningi.Le datha futhi iphakamisa ukuthi ukwanda kwe-leucine yezokudla kungase kunikeze isengezo ekulawuleni ukumelana ne-insulin ehlobene nokukhuluphala.