I-L-Leucine Cas: 61-90-5 99% Impushana emhlophe
| Inombolo Yekhathalogi | XD90304 |
| Igama Lomkhiqizo | I-L-Leucine |
| I-CAS | 61-90-5 |
| I-Molecular Formula | I-C6H13NO2 |
| Isisindo samangqamuzana | 131.17292 |
| Imininingwane Yesitoreji | I-ambient |
| Ikhodi Yentela Evumelanisiwe | 29224985 |
Ukucaciswa Komkhiqizo
Ngokufanayo namanye amagciwane e-RNA anomucu omuhle, ama-tombusvirus aphindaphindwa yi-membrane-bound viral replicase complex (VRC).I-VRC iqukethe i-RNA polymerase (RdRp encike kugciwane e-p92), i-viral p33 RNA chaperone, kanye namaphrotheni ambalwa akhethwe ngokubambisana.Ukuze ibe i-RdRp esebenzayo ngemva kokuhumusha kwayo, iphrotheni ye-p92 yokuphindaphinda kufanele ifakwe ku-VRC, kulandele ukwenziwa kwayo kusebenze.Sike sabonisa ngaphambilini ekuhlolweni okusekelwe ku-yeast-free-yeast-based assay ukuthi ukwenziwa kusebenze kwe-Tomato bushy stunt virus (TBSV) RdRp kudinga i(zi)nto ezincibilikayo.Kulesi sihloko, sihlonza iphrotheni yokwethuka kokushisa kweselula engu-70 (Hsp70) njengento yokusingatha ekhethiwe edingekayo ukuze kwenziwe kusebenze i-N-terminally truncated recombinant TBSV RdRp.Ngaphezu kwalokho, ukuvinjwa okusekelwe ku-molecule encane yokusebenza kwe-Hsp70 kuvimbela ukuhlanganiswa kwe-RNA yi-tombusvirus RdRp in vitro.Ngaphezu kwalokho, sibonisa ukuthi i-phospholipids engathathi hlangothi, okungukuthi, i-phosphatidylethanolamine (PE) ne-phosphatidylcholine (PC), ithuthukisa ukusebenza kwe-RdRp ku-vitro.Ngokuphambene, i-phosphatidylglycerol (PG) ikhombisa umthelela oqinile futhi olawulayo wokuvinjelwa ekusebenziseni i-in vitro RdRp.Siphinde futhi sibonise ukuthi i-PE ne-PC ivuselela ukusebenzisana kwe-RdRp-viral plus-strand RNA [(+)RNA], kuyilapho i-PG ivimbela ukubophezela kwe-RNA yegciwane ku-RdRp.Ngokusekelwe kuzindima ezikhuthazayo nezivimbelayo zama-phospholipid ahlukahlukene ekwenzeni kusebenze i-tombusvirus RdRp, siphakamisa ukuthi ukwakheka kwe-lipid kolwelwesi oluqondiswe kuma-subcellular kungase kusetshenziswe ama-tombusviruses ukulawula ukuhlanganiswa kwe-VRC okusha phakathi nokutheleleka.I-RNA polymerase encike ku-RNA esekelwe kugciwane (RdRp), enesibopho sokuhlanganisa inzalo yegciwane le-RNA kumaseli athelelekile amagciwane e-RNA anemicu eminingana eqondile, ayisebenzi ekuqaleni.Leli su kungenzeka ligweme ukuhlanganiswa kwe-RNA yegciwane ku-cytosol okungaholela ngokushesha ekufakweni kwezimpendulo ze-antiviral zeselula ezibangelwa i-RNA.Ngesikhathi sokuhlanganisa inkimbinkimbi yokuphindaphinda eboshwe ulwelwesi, i-RdRp yegciwane yenziwa isebenze ngenqubo engaqondakali kahle eyenza i-RdRp ikwazi ukuhlanganiswa kwe-RNA.Ngokusebenzisa i-TBSV RdRp, sibonisa ukuthi i-Hsp70 chaperone yeselula ekhethiwe kanye nama-phospholipids angathathi hlangothi enza kusebenze i-RdRp ku-vitro.Ngokuphambene, i-phosphatidylglycerol (PG) inomphumela onamandla wokuvinjwa kwe-in vitro RdRp kanye nokusebenzisana kwe-RdRp-viral RNA, okuphakamisa ukuthi i-microdomain ye-membranous ezungeze i-RdRp ithinta kakhulu amandla ayo okuhlanganiswa kwe-RNA.Ngakho-ke, ukwenziwa kusebenze kwe-viral RdRp kungenzeka kuncike ezingxenyeni eziningi zokusingatha kumaseli athelelekile.
I-4-Nitrophenyl-N-acetyl-β- D-glucosaminide iyi-substrate ewusizo yokuhlolwa kwe-colorimetric okusheshayo komsebenzi we-N-acetyl-b-glucosaminidase kumchamo womuntu.I-Chromogenic β-Glucosaminidase substrate ikhiqiza isixazululo esiphuzi lapho iqhekeka, esetshenziswa ikakhulukazi kumvubelo nezibundi.
