I-D-Luciferin Cas: 2591-17-5 99% Okumhlophe-kumhlophe kuya kumpuphu ophuzi nbsp I-BEETLE LUCIFERIN
| Inombolo Yekhathalogi | XD90248 |
| Igama Lomkhiqizo | D-Luciferin |
| I-CAS | 2591-17-5 |
| I-Molecular Formula | I-C11H8N2O3S2 |
| Isisindo samangqamuzana | 280.323 |
| Imininingwane Yesitoreji | -15 kuya ku-20 ° C |
| Ikhodi Yentela Evumelanisiwe | 29342080 |
Ukucaciswa Komkhiqizo
| Okuqukethwe Amanzi | Ubukhulu.2.0% |
| I-Turbidity | Ubukhulu 2.0 NTU |
| Ukujikeleza okuqondile okubonakalayo | -36 kuya ku-32 |
| Ukubukeka | Okumhlophe kuya kokuphuzi |
| I-Purity HPLC | Ubuncane 99% |
| I-molar extinction coefficient | iminithi engu-17900 L/(mol cm) |
Isingeniso: I-D-luciferin iyi-substrate ye-adenosine triphosphate (ATP)-encike ku-bioluminescence reaction.Umgomo we-bioluminescence ukuthi i-luciferin i-oxidized yi-luciferase phambi kwe-ATP ne-oxygen.Ifomula yokusabela kwamakhemikhali imi kanje: ATP+D-Luciferin+O2→Oxyluciferin+AMP+PPi+O2+Light.
Indlela yokusebenza: Indlela yokusebenza ye-D-luciferin yukuthi ngaphansi kwesenzo se-ATP ne-luciferase, i-luciferin (i-substrate) ingafakwa i-oxidized ukuze ikhiphe ukukhanya.Uma i-luciferin ingaphezu kwamandla, inani lama-photons akhiqizwayo lihlotshaniswa kahle nokuhlushwa kwe-luciferase.
Isicelo: I-D-luciferin iyi-substrate ye-adenosine triphosphate (ATP)-encike ku-bioluminescence reactions.Ukusabela kwe-bioluminescence kwe-luciferin/luciferase kuvame ukusetshenziselwa ukutholwa kwe-ATP, ama-metabolites angaguqulwa abe yi-ATP (njenge-AMP, i-ADP, i-cAMP), nama-enzyme angakwazi ukukhiqiza i-ATP (njenge-creatine kinase, njll.), ukusabela kwe-bioluminescence kungasetshenziswa.Kusebenza ekutholweni kwezinhlobonhlobo zezinto eziphilayo.
Umsebenzi Wezinto Eziphilayo: I-D-Luciferin (i-Firefly luciferin) i-substrate ethandwayo ye-bioluminescence phambi kwe-ATP ukuze isetshenziswe ku-luciferase-based bioluminescence imaging kanye nokuhlolwa kwe-cell-based high-throughput.
Izifundo ze-in vitro: I-D-luciferin isabela nge-luciferase, i-ATP nomoya-mpilo ukuze ikhiphe ukukhanya, okutholwa yifilimu yezithombe ebucayi ukuze kubonakale ngeso lengqondo amasosha omzimba abopha i-alkaline phosphatase.
Izifundo ze-in vivo: ukusetshenziswa kwe-D-luciferin substrates kanye ne-firefly luciferase kugcina ukusebenzisana kwe-tumor-host immune kumodeli yegundane elikwazi ukumelana nomdlavuza we-ovarian, njengoba izinhlelo ze-bioluminescence zizwela kakhulu ekukhuleni kwesimila kunemiyalelo yokuzuza isisindo somzimba.
