dCTP, 2′-Deoxycytidine-5′-triphosphoric acid disodium salt Cas:102783-51-7 White Powder
| Inombolo Yekhathalogi | XD90584 |
| Igama Lomkhiqizo | dCTP, 2'-Deoxycytidine-5'-triphosphoric acid disodium usawoti |
| I-CAS | 951-78-0 |
| I-Molecular Formula | I-C9H12N2O5 |
| Isisindo samangqamuzana | 228.20 |
| Imininingwane Yesitoreji | -20 °C |
| Ikhodi Yentela Evumelanisiwe | 29349990 |
Ukucaciswa Komkhiqizo
| Ukubukeka | Impushana Emhlophe |
| Isivivinyo | 99% |
Amaseli e-Eukaryotic aqukethe ibhalansi ethambile yamanani amancane ama-deoxyribonucleoside triphosphates (dNTPs), anele imizuzu embalwa yokuphindaphinda kwe-DNA.Kokubili ukuntula kanye nensalela ye-dNTP eyodwa kungase kubangele ukukhuphuka kwamazinga okuguquka, ukulungiswa kwe-DNA okuyiphutha noma ukuncipha kwe-DNA ye-mitochondrial.I-dNTP ivamise ukulinganiswa ngokuhlolwa kwe-enzymatic lapho ukufakwa kwe-dATP enemisebe (noma i-dTTP enemisebe ekuhlolweni kwe-dATP) kuma-oligonucleotide athile okwenziwa yi-DNA polymerase ilingana nokuhlangana kwe-dNTP engaziwa.Sithola ukuthi i-Klenow DNA polymerase evame ukusetshenziswa ingase ithathele i-ribonucleotide ehambisanayo ye-dNTP engaziwa eholela kwezinye izimo ekulinganisweni ngokweqile kwama-dNTP.Manje sichaza izimo zokuhlola ze-dNTP ngayinye ezigwema ukufakwa kwe-ribonucleotide.Mayelana nokuhlolwa kwe-dTTP kanye ne-dATP kwanele ukunciphisa ukugxila kwe-enzyme ye-Klenow kanye nokubhalwe ukuthi dATP (noma i-dTTP);ku-dCTP ne-dGTP kwadingeka ukuthi sishintshe i-enzyme ye-Klenow sifake i-Taq DNA polymerase noma i-Thermo Sequenase.Siphakamisa ukuthi kweminye imibiko yangaphambili ukufakwa kwe-ribonucleotide kungase kubangele amanani aphezulu kakhulu e-dGTP ne-dCTP.
