I-Deoxyribonucleic acids, isidoda sezinhlanzi Cas:100403-24-5
| Inombolo Yekhathalogi | XD90579 |
| Igama Lomkhiqizo | I-Deoxyribonucleic acids, isidoda sezinhlanzi |
| I-CAS | 100403-24-5 |
| I-Molecular Formula | - |
| Isisindo samangqamuzana | - |
| Imininingwane Yesitoreji | 2 kuya ku-8 °C |
| Ikhodi Yentela Evumelanisiwe | 29349990 |
Ukucaciswa Komkhiqizo
| Ukubukeka | Impushana emhlophe |
| Isivivinyo | 99% |
Izindlela zamangqamuzana e-pathogenic ezifweni zesibindi sokucindezelwa yi-cirrhotic ezifana ne-hepatitis C virus (HCV), i-autoimmune hepatitis (AIH) kanye ne-primary biliary cirrhosis (PBC) azibonakali kahle.Izakhi zofuzo ezivezwe ngokuhlukile zivame ukubaluleka ku-pathogenesis yesifo.I-Suppression subtractive hybridization (SSH) iyindlela ebanzi yofuzo ethuthukisa imibhalo evezwe ngokwehlukana ye-mRNA.Besihlose ukwenza ukuqaphela okunoveli kokuhluka kofuzo lwesisho ku-cirrhosis sisebenzisa i-SSH ehlanganiswe ne-quantitative real-time reverse reverse transcriptase polymerase chain reaction (RT-PCR).I-transcriptome yesibindi ku-HCV cirrhosis, i-AIH cirrhosis, i-PBC, kanye nezicubu zesibindi ezingenalutho zihlolwe yi-SSH.Ama-clones ahambisanayo e-DNA (cDNA) aphinde ahlolwa ukuze abonise umehluko ngokuxutshwa kwe-dot-blot bese alandelana.Inkulumo yofuzo ekhethiwe yalinganiswa nge-RT-PCR yesikhathi sangempela.Ukulandela i-SSH, ama-clones angu-694 aphinde ahlolwa ukuze abonise ukuhluka kwezakhi zofuzo, lapho angu-145 alandelana futhi atholakala ephuma ezakhini zofuzo ezihlukene ezingu-89.Ama-clones ayisikhombisa abenofuzo olufanayo kuphela ngokulandelana kwethegi yokulandelana eshiwo (EST) engenawo umsebenzi owaziwayo.Ukubonakaliswa okulawulwa phezulu kwezakhi zofuzo ezine kwaqinisekiswa yi-RT-PCR yesikhathi sangempela: i-transmembrane 4 ilungu lomndeni omkhulu 3 (tetraspanin CO-029) kuzo zonke izinhlobo ze-cirrhosis, i-hedgehog interacting protein (HIP) ku-AIH cirrhosis kanye ne-chitinase 3-like-1 (HC gp-39 noma ykl-40) kanye ne-arginine-glutamic acid ephinda (RERE) ku-HCV cirrhosis.I-RERE gene polymorphisms kanye nokuhlukahluka kwe-splice kwabonwa kuzo zonke izicubu ezihloliwe.I-Tetraspanin CO-029 up-regulation yenziwe yasendaweni ngokuyinhloko kumaseli e-bile ductular.Sengiphetha, ukubonwa kwenoveli kokuhluka kwezakhi zofuzo ku-cirrhosis yomuntu kwenziwa kusetshenziswa i-SSH njengethuluzi eliyinhloko lokuthola.Ikakhulukazi, izifundo ezengeziwe zofuzo lwe-RERE kanye nemikhiqizo yayo ku-HCV yesifo sesibindi esihlobene nazo ziqinisekisiwe.
