I-GSH Cas: 70-18-8 98% I-crystalline powder emhlophe
| Inombolo Yekhathalogi | XD90227 |
| Igama Lomkhiqizo | I-GSH |
| I-CAS | 70-18-8 |
| I-Molecular Formula | I-C10H17N3O6S |
| Isisindo samangqamuzana | 307.323 |
| Imininingwane Yesitoreji | 2 kuya ku-8 °C |
| Ikhodi Yentela Evumelanisiwe | 29309098 |
Ukucaciswa Komkhiqizo
| Ukucaciswa Komkhiqizo | |
| I-Melting Point | 185°C |
| Ibanga | I-Pharma Grade |
| Izinsimbi ezinzima | 10ppm ubuningi |
| I-Arsenic | 2ppm ubuningi |
| Ukuhlonza | I-IR : Iyafana ne-Reference Spectrum |
| Ukulahlekelwa Ekumisweni | 0.5% ubuningi |
| Izinga Lokushisa Lokugcina | +20 ° C |
| Isisindo samangqamuzana | 612.63 |
| Isivivinyo | 98.0% iminithi |
| Ukujikeleza kwe-Optical | -15.5°- -17.5 deg |
| Izinsalela ekushiseni | 0.1% ubuningi |
| Ukucaca Kwesixazululo | Icacile futhi ayinambala |
| Ukubukeka | I-crystalline powder emhlophe |
| Into Ehlobene | Isamba: 2.0% ubuningi, GSSG:1.5% ubuningi |
Iphrotheni ye-ORF45 ye-Kaposi's sarcoma-associated herpesvirus (KSHV) i-gammaherpesvirus-specific immediate-early tegument protein.Ucwaningo lwethu lwangaphambilini luveze izindima zalo ezibalulekile kuzo zombili izigaba zakuqala nezakamuva zokutheleleka nge-KSHV.Kulolu cwaningo, senze inhlolovo yokusebenzisana kwe-ORF45 sisebenzisa iphaneli yamasosha omzimba e-monoclonal.Ngaphezu kwamaprotheni ahlonzwe ngaphambilini e-extracellular regulated kinase (ERK) kanye ne-p90 ribosomal S6 kinase (RSK), sithole amanye amaprotheni amaningana ahlanganisiwe, okuhlanganisa avelele angu-∼38 kDa kanye ~130 kDa.I-mass spectrometry iveze ukuthi iphrotheni engu-38-kDa iyigciwane i-ORF33 kanye nephrotheni engu-130-kDa i-USP7 yeselula (ubiquitin-specific protease 7).Senze imephu yesizinda esibophezelayo se-ORF33 ku-carboxyl-terminal 19 amino acids (aa) egcinwe kakhulu ye-ORF45 kanye nesizinda esibophayo se-USP7 ku- motif yesivumelwano esibikiwe endaweni emaphakathi ye-ORF45.Sisebenzisa i-immunofluorescence staining, sibone ukuhlanganisa kwe-ORF45 nge-ORF33 noma i-USP7 kokubili ngaphansi kwama-ion we-condit amagciwane kanye namaseli anegciwane le-KSHV.Ngaphezu kwalokho, siqaphele ukushintshwa okuncike ku-ORF45 kwengxenye ye-ORF33/USP7 ukusuka ku-nucleus kuya ku-cytoplasm.Sithole ukuthi i-ORF45 ibangele ukwanda kokuqoqwa kwamaprotheni e-ORF33 aqedwa uma ngabe isizinda esibophezelayo se-ORF33- noma i-USP7 ku-ORF45 sisusiwe.Ngaphezu kwalokho, ukususwa kwe-carboxyl terminus elondoloziwe ye-ORF45 kugenome ye-KSHV kwehlise kakhulu izinga le-ORF33 amaprotheni kumaseli atheleleke nge-KSHV futhi kwaqeda ukukhiqizwa kwama-virion enzalo.Ngokuhlangene, imiphumela yethu ayivezi nje izingxenye ezintsha ze-ORF45 interactome, kodwa futhi ibonisa ukuthi ukusebenzisana phakathi kwalawa maprotheni kubalulekile ekuphindaphindeni kwe-KSHV.I-KSHV ORF45 iyiphrotheni enemisebenzi eminingi edingekayo ekuphindaphindeni kwe-KSHV lytic, kodwa izindlela eziqondile i-ORF45 eyenza ngazo imisebenzi yayo ebalulekile azicacile.Ucwaningo lwethu lwangaphambilini luveze ukuthi yonke i-ORF45 pr otein kumaseli ikhona kuma-high-molecule-weight complexes.Ngakho-ke sifune ukuveza i-interomeome ye-ORF45 ukuze sinikeze imininingwane ngezindima zayo phakathi nokuphindaphinda kwe-lytic.Sisebenzisa iphaneli yamasosha omzimba e-monoclonal, sihlole i-ORF45 interactome kumaseli anegciwane le-KSHV.Sihlonze ozakwethu ababili abasha ababophayo be-ORF45: iphrotheni yegciwane i-ORF33 kanye ne-ubiquitin-specific protease 7 (USP7).Siphinde sibonisa ukuthi ukusebenzisana phakathi kwe-ORF45 ne-ORF33 kubalulekile ekukhiqizeni ngempumelelo izinhlayiya zegciwane le-KSHV, okuphakamisa ukuthi ukuphazamiseka okuhlosiwe kulokhu kusebenzisana kungase kumelele isu elinoveli lokuvimbela ukuphindaphinda kwe-KSHV lytic.
