I-Hygromycin B CAS:31282-04-9 Buff powder
| Inombolo Yekhathalogi | XD90374 |
| Igama Lomkhiqizo | Hygromycin B |
| I-CAS | 31282-04-9 |
| I-Molecular Formula | I-C20H37N3O13 |
| Isisindo samangqamuzana | 527.52 |
| Imininingwane Yesitoreji | 2 kuya ku-8 °C |
| Ikhodi Yentela Evumelanisiwe | 2941900000 |
Ukucaciswa Komkhiqizo
| Izinsimbi ezinzima | 20mg/kg ubuningi |
| pH | 7-9.5 |
| Isivivinyo | 99% |
| Ukulahlekelwa Ekumisweni | 5% ubuningi |
| umsebenzi | 950u/mg iminithi |
| I-ammonium | 1% ubuningi |
| Umlotha one-sulfate | 5% ubuningi |
| Ukubukeka | Buff powder |
| I-Purity TLC | >90% |
Ukuguga okulandelanayo kwamangqamuzana imvubelo kuvame ukusetshenziswa njengemodeli yokuguga kwamaseli omuntu we-post-mitotic.Iyeast Saccharomyces cerevisiae etshalwe ku-glucose phambi kwe-ammonium sulphate isetshenziswa kakhulu ocwaningweni lokuguga imvubelo.Sihlaziye ukuguga okulandelanayo kweyeast ye-Hansenula polymorpha ekhule ngaphansi kwezimo ezidinga i-peroxisome metabolism ukuze ikhule.Isikhathi sokuphila sokulandelana kwe-H. polymorpha sithuthukiswa kakhulu lapho amaseli ekhuliswa nge-methanol noma i-ethanol, ehlanganiswa ama-enzyme e-peroxisome, ngokuhlobene nokukhula kushukela. lokho akudingi i-peroxisomes.Ukuphila okufushane kwe-H. polymorpha ku-glucose ngokuyinhloko kungenxa ye-acidification emaphakathi, kuyilapho i-ROS cishe ayinayo indima ebalulekile.Ukukhula kwamaseli ku-methanol/methylamine esikhundleni se-methanol/ammonium sulphate kuholele ekuthuthukisweni okuqhubekayo kwesikhathi sokuphila.Lokhu bekungahlobene ne-acidification emaphakathi.Sibonisa ukuthi i-oxidation ye-methylamine nge-peroxisomal amine oxidase ezimeni zendlala ye-carbon inesibopho sokunwetshwa kwempilo.Umkhiqizo we-methylamine oxidation we-formaldehyde uphinde ufakwe i-oxidized okuholela ekukhiqizeni kwe-NADH, okunomthelela ekwandiseni ukukhiqizwa kwe-ATP nokwehliswa kwamazinga e-ROS esigabeni sokunganyakazi.Ngaphezu kwalokho, ithuba lokukhiqiza i-NADH ezimeni zokulamba kwekhabhoni ngomthombo we-nitrogen ophilayo lisekela ukunwetshwa okwengeziwe kwesikhathi sokuphila kweseli.Ngenxa yalokho, ukuhunyushwa kokuhlaziywa kwe-CLS ku-yeast kufanele kufake imiphumela engaba khona esimweni samandla seseli.
