Kanamycin A Sulfate CAS:25389-94-0 I-crystalline powder emhlophe
| Inombolo Yekhathalogi | XD90363 |
| Igama Lomkhiqizo | I-Kanamycin A sulfate |
| I-CAS | 25389-94-0 |
| I-Molecular Formula | C18H36N4O11 · H2O4S |
| Isisindo samangqamuzana | 582.58 |
| Imininingwane Yesitoreji | 15 kuya ku-30 °C |
| Ikhodi Yentela Evumelanisiwe | 29419000 |
Ukucaciswa Komkhiqizo
| Ibanga | USP |
| pH | 6.5-8.5 |
| I-endotoxin yebhaktheriya | <0.60EU/mg |
| Ukulahlekelwa Ekumisweni | 4.0% ubuningi |
| Ukuncibilika | Incibilika ngokukhululeka emanzini, ayincibiliki ku-acetone ku-ethyl acetate naku-benzene |
| Isivivinyo | > 750ug/mg |
| Umlotha one-sulphate | 11 - 17.7% |
| Izinsalela ekushiseni | <1.0% |
| Ukujikeleza okuqondile okubonakalayo | +112 - +123 |
| Izincibilikisi Ezisele | I-Ethanol 2000ppm Max |
| Ukungcola Okuphelele | 3.0% ubuningi |
| Ukubukeka | I-crystalline powder emhlophe |
| Usuku Lokukhiqiza | I-TBC |
| I-Kanamycin B | 1.5% ubuningi |
| Noma yikuphi ukungcola okungaziwa | 0.45% ubuningi |
| Umkhawulo weMicrobial | 25cfu/g Ubukhulu |
Amaprotheni/ama-peptide e-antimicrobial adonse ukunaka okukhulu ngenxa yokusebenzisa kwawo okungaba khona endaweni yezimboni.Ocwaningweni lwamanje, iphrotheni ye-antimicrobial eshisayo (i-BSAMP) yahlanzwa ku-culture supernatant ye-Bacillus subtilis FB123 nge-ammonium sulfate precipitation, i-gel chromatography ku-Sephacryl S-200 High Resolution, kanye ne-ion exchange chromatography kukholomu ye-DEAE Sepharose Flow Flow.Isisindo samangqamuzana e-BSAMP ehlanziwe sasingu-54 kDa, njengoba kulinganiselwa nge-sodium dodecyl sulfate-polyacrylamide gel electrophoresis kokubili lapho ingekho kanye nokuba khona kwe-β-mercaptoethanol.Iphoyinti layo le-isoelectric lanqunywa ukuthi libe ngu-5.24 nge-isoelectric focusing electrophoresis.I-Periodic acid-Schiff staining yembula i-BSAMP ukuthi iyi-glycoprotein.Umsebenzi omkhulu utholwe ku-pH 6.0, nomsebenzi omkhulu ongaphezu kuka-79% ogcinwe ku-pH 3.0-5.0 kanye ne-pH 7.0-9.0, ngokulandelanayo.I-BSAMP iboniswe ukuthi ikwazi ukushisisa kakhulu, njengoba umsebenzi wayo awuzange ushintshe ngokusobala ngemva kokwelashwa ku-100 °C.Nokho, yayizwela ngokwengxenye kuma-papain, trypsin, nama-alkali protease.Ekugcineni, iphrotheni yebhaktheriya yabonisa umsebenzi obanzi we-antimicrobial ngokumelene nezinambuzane eziningana ze-pathogenic.Lokhu okutholakele kuphakamise ukuthi i-BSAMP kufanele ithuthukiswe futhi njenge-ejenti yemvelo elwa namagciwane ekuvimbeleni izifo ekulimeni kwezilwane zasemanzini kanye nezolimo.
