L-Lactate dehydrogenase CAS:9001-60-9
| Inombolo Yekhathalogi | XD90395 |
| Igama Lomkhiqizo | I-L-Lactate dehydrogenase |
| I-CAS | 9001-60-9 |
| I-Molecular Formula | - |
| Isisindo samangqamuzana | - |
| Ikhodi Yentela Evumelanisiwe | 35079090 |
Ukucaciswa Komkhiqizo
| Ukubukeka | Impushana emhlophe |
Ukuhlaziywa kwemicimbi ye-proteome-wide phosphorylation kuseyinselelo enkulu yokuhlaziya ngenxa yenkimbinkimbi enkulu yamanethiwekhi we-protein phosphorylation.Kulo msebenzi, sihlola ukuhambisana kwe-Lys-N, Lys-C, kanye ne-trypsin mayelana nekhono labo lokufaka isandla ekuhlaziyweni komhlaba wonke kwe-phosphoproteome.Inguqulo ecwengisisiwe yokushintshisana kwe-cation eqinile ye-pH ephansi iye yasetshenziswa ukuze kuhlukaniswe kahle i-N-terminally acetylated, phosphorylated, nama-peptide angashintshiwe.Ingqikithi yama-phosphopeptides angadingeki angu-5036 angabonakala ngenani lokutholwa okungamanga le-<1% kusuka ku-1 mg wamaprotheni kusetshenziswa inhlanganisela yama-enzyme amathathu.Idatha yethu iveze ukuthi ukugqagqana phakathi kwamasethi edatha ye-phosphopeptide ekhiqizwe ngama-protease ahlukene kwakuseceleni, kuyilapho ukugqagqana phakathi kwamasethi edatha amabili e-tryptic akhiqizwe ngendlela efanayo kutholwe okungenani kuphakeme izikhathi ezi-4.Ngale ndlela, ukusetshenziswa okufanayo kwe-Lys-N ne-trypsin kwenze ukuba kukhuphuke ngo-72% inani lama-phosphopeptides atholiwe njengoba kuqhathaniswa d ne-trypsin iyodwa, kanti ukuhlolwa kokuphindaphinda kwe-trypsin kuholele ekwenyukeni okungama-25%.Ngakho-ke, lapho sigxile kuphela kudatha ye-trypsin ne-Lys-N, sihlonze ama-phosphopeptides angadingeki angama-4671.Ukuhlaziywa okwengeziwe kwezingosi ezitholiwe kubonise ukuthi i-Lys-N kanye nesethi yedatha ye-trypsin inothisiwe kuma-phosphorylation motifs ahluke kakhulu, okufakazela futhi ukuthi izindlela ze-multiprotease zibaluleke kakhulu ekuhlaziyweni kwe-phosphoproteome.
