I-L-Methionine Cas: 63-68-3 99% I-crystalline powder emhlophe
| Inombolo Yekhathalogi | XD90287 |
| Igama Lomkhiqizo | I-L-Methionine |
| I-CAS | 63-68-3 |
| I-Molecular Formula | I-C5H11NO2S |
| Isisindo samangqamuzana | 149.21134 |
| Imininingwane Yesitoreji | I-ambient |
| Ikhodi Yentela Evumelanisiwe | 29304010 |
Ukucaciswa Komkhiqizo
| Isivivinyo | 98% - 101.5% |
| Ukubukeka | Amakristalu amhlophe noma i-crystalline powder |
| Isiphetho | |
| Ukuhlonza | Ihlangabezana nemfuneko |
| pH | 5.6 - 6.1 |
| Ukulahlekelwa Ekumisweni | ≤ 0.3% |
| I-Sulfate (SO4) | ≤ 0.03% |
| Insimbi | ≤ 0.003% |
| Izinsalela ekushiseni | ≤ 0.4% |
| I-Chloride | ≤ 0.05% |
| Insimbi Enzima | ≤ 0.0015% |
| Ukuhlanzeka kweChromotograhic | Akutholakali ngaphezu kuka-2.0% wokungcola okuphelele |
| Ukuphenduka okuqondile [ α ] D 2 5 | +22.4º ~ +24.7º |
Ucwaningo lwethu lwangaphambilini luveze izindima zalo ezibalulekile kuzo zombili izigaba zakuqala nezakamuva zokutheleleka nge-KSHV.Kulolu cwaningo, senze inhlolovo yokusebenzisana kwe-ORF45 sisebenzisa iphaneli yamasosha omzimba e-monoclonal.Ngaphezu kwamaprotheni ahlonzwe ngaphambilini e-extracellular regulated kinase (ERK) kanye ne-p90 ribosomal S6 kinase (RSK), sithole amanye amaprotheni amaningana ahlanganisiwe, okuhlanganisa avelele angu-∼38 kDa kanye ~130 kDa.I-mass spectrometry iveze ukuthi iphrotheni engu-38-kDa iyigciwane i-ORF33 kanye nephrotheni engu-130-kDa i-USP7 yeselula (ubiquitin-specific protease 7).Senze imephu yesizinda esibophezelayo se-ORF33 ku-carboxyl-terminal 19 amino acids (aa) egcinwe kakhulu ye-ORF45 kanye nesizinda esibophayo se-USP7 ku- motif yesivumelwano esibikiwe endaweni emaphakathi ye-ORF45.Sisebenzisa i-immunofluorescence staining, siye sabona ukuhlanganiswa kwe-ORF45 ne-ORF33 noma i-USP7 kokubili ngaphansi kwezimo ezithathelwanayo kanye namaseli anegciwane le-KSHV.Ngaphezu kwalokho, siqaphele ukushintshwa okuncike ku-ORF45 kwengxenye ye-ORF33/USP7 ukusuka ku-nucleus kuya ku-cytoplasm.Sithole ukuthi i-ORF45 ibangele ukwanda kokuqoqwa kwamaprotheni e-ORF33 aqedwa uma ngabe isizinda esibophezelayo se-ORF33- noma i-USP7 ku-ORF45 sisusiwe.Ngaphezu kwalokho, ukususwa kwe-carboxyl terminus elondoloziwe ye-ORF45 kugenome ye-KSHV kwehlise kakhulu izinga le-ORF33 amaprotheni kumaseli atheleleke nge-KSHV futhi kwaqeda ukukhiqizwa kwama-virion enzalo.Ngokuhlangene, imiphumela yethu ayivezi nje izingxenye ezintsha ze-ORF45 interactome, kodwa futhi ibonisa ukuthi ukusebenzisana phakathi kwalawa maprotheni kubalulekile ekuphindaphindeni kwe-KSHV.I-KSHV ORF45 iyiphrotheni enemisebenzi eminingi edingekayo ekuphindaphindeni kwe-KSHV lytic, kodwa izindlela eziqondile i-ORF45 eyenza ngazo imisebenzi yayo ebalulekile azicacile.Ucwaningo lwethu lwangaphambilini lwembula ukuthi wonke amaprotheni e-ORF45 kumaseli akhona kuma-high-molecule-weight complexes.Ngakho-ke sizame ukuveza i-interomeome ye-ORF45 ukuze sinikeze imininingwane ngezindima zayo phakathi nokuphindaphinda kwe-lytic.Sisebenzisa iphaneli yamasosha omzimba e-monoclonal, sihlole i-ORF45 interactome kumaseli anegciwane le-KSHV.Sihlonze ozakwethu ababili abasha ababophayo be-ORF45: iphrotheni yegciwane i-ORF33 kanye ne-ubiquitin-specific protease 7 (USP7).Siphinde sibonisa ukuthi ukusebenzisana phakathi kwe-ORF45 ne-ORF33 kubalulekile ekukhiqizeni ngempumelelo izinhlayiya zegciwane le-KSHV, okuphakamisa ukuthi ukuphazamiseka okuhlosiwe kulokhu kusebenzisana kungase kumelele isu elinoveli lokuvimbela ukuphindaphinda kwe-KSHV lytic.
