(S)-2-Amino-4-hydroxybutanoic acid Cas: 672-15-1
| Inombolo Yekhathalogi | XD90286 |
| Igama Lomkhiqizo | (S) -2-Amino-4-hydroxybutanoic acid |
| I-CAS | 672-15-1 |
| I-Molecular Formula | C4H9NO3 |
| Isisindo samangqamuzana | 119.11916 |
| Imininingwane Yesitoreji | I-ambient |
| Ikhodi Yentela Evumelanisiwe | 29225000 |
Ukucaciswa Komkhiqizo
| Isivivinyo | 99% |
| Ukubukeka | Impushana emhlophe |
Ucwaningo lwamanje luchaza indlela elula nephumelelayo esebenzisa i-high performance liquid chromatography (HPLC) ehlanganiswe nokutholwa kwe-fluorescence ukuze kunqunywe amapharamitha we-kinetic wokuthatha kwe-glutamate esicutshini sezinzwa.Izicubu ze-retina ezitholwe kumatshwele anezinsuku ezingu-7 ubudala zafakwa ngokugxila okwaziwayo kwe-glutamate (50-2000 μM) imizuzu engu-10, futhi amazinga e-o-phtaldehyde (OPA) -i-neurotransmitter ephuma ku-incubation medium akalwa.Ngokuhlola umehluko phakathi kokugxila kokuqala nokokugcina kwe-glutamate phakathi nendawo, indlela yokuthatha egcwalayo ibonakaliswe (K(m)=8.2 kanye ne-V(max)=9.8 nmol/mg protein/min).Lesi silinganiso sasincike kakhulu kwi-sodium- kanye nezinga lokushisa, sisekela ngokuqinile ukuthi indlela yokunciphisa ukugxila ithathwa ngabathuthi abasondelene kakhulu.Ukwengeza kulokhu, imiphumela yethu iphinde yabonisa ukuthi i-zinc chloride (i-inhibitor ye-glutamate/aspartate transporters) ibangele ukwehla okuncike ekugxiliseni ekuthathweni kwe-glutama te, okubonisa ukucaciswa kwendlela yethu.Sekukonke, umsebenzi wamanje ubonisa enye indlela yokuhlola ukutholwa kwe-glutamate ezicutshini zezinzwa kusetshenziswa i-HPLC.Le ndlela ingaba ithuluzi elibalulekile lezifundo ezihlotshaniswa nokubonakaliswa kokuguqulwa kwamaminithi ekuthuthweni kwe-glutamate okuhlobene nokulimala kwesistimu yezinzwa ezimaphakathi.
