Imikhiqizo

Kwasungulwa ukuhlolwa kwamabala okulula, okuvumela ukulinganiswa kwe-microalbuminuria.Ukuhlola kwenziwe ngokweziqondiso ze-ISO 15189. Umchamo wabonwa emicu ye-cellulose acetate futhi wagcotshwa kusetshenziswa odayi abahlukene abazwela amaprotheni (i-nigrosin, i-Coomassie Blue R-250, i-amido emnyama).Ukuqina kombala wamabala anamabala alinganiswa kusetshenziswa isiteshi se-Kodak Image 450. Ukuzwela kokuhlaziya kwendlela esekelwe e-Coomassie Blue (18 mg/L) kwakungcono kunaleyo ye-nigrosin (50 mg/ L) noma i-amido emnyama (100 mg/L ) izindlela ezisekelwe.I-coefficient esetshenziswayo yokuhluka (i-CV) kanye ne-CV ephakathi kokusetshenziswa kokuhlolwa okuluhlaza okwesibhakabhaka kwe-Coomassie, ngokulandelana, bekungu-8.4% no-9.7% (50 mg/L), kanye no-3% no-4.5% (400 mg/L).Ku-nigrosin, le datha, ngokulandelanayo, yayingu-8.4 no-9.4 (50 mg/L), kanye no-3.4 kanye no-6.4% (400 mg/L).I-Coomassie Blue ibonise ukukhetha okubophayo okuncamelayo ku-albumin.Indlela itholwe inomugqa phakathi kuka-20 no-600 mg/L.Ukuhlobana okuhle (r2 = 0.89) kutholwe phakathi kwezilinganiso ezisekelwe ku-Coomassie Blue kanye ne-immunonephelometric.I-albumin ye-immuno-unreactive (elungiselelwe ukwelashwa kwe-protease) ingatholwa ngokuhlolwa kwendawo, okunikeza inzuzo yendlela eqhathaniswa nokuhlolwa kwe-immunochemical.Imvula ye-Ammonium sulphate ingase ikhulise ukucaciswa kokuhlolwa ngokuqeda imiphumela yamaketanga okukhanya amahhala. Indlela echaziwe ilula kakhulu futhi ishibhile kakhulu, okuyenza ifanelekele izinhlelo zokuhlola, ikakhulukazi emazweni omhlaba wesithathu.