Imikhiqizo

Ukuze kucaciswe inqubo yokuqala ye-dentin mineralization, umphumela ovimbelayo we-1-hydroxyethylidene-1,1-bisphosphonate (HEBP) kumaminerali we-dentin waphenywa.Amagundane (100 g) ajovwe ngaphansi kwesikhumba nge-HEBP (8 mg P/kg) ye-7 noma i-14 d, futhi ama-incisors acutshungulwa i-Ca histochemistry kanye/noma i-electron microscopy.Ama-incisors alashwe nge-HEBP abonise imigqa ehlukile efana nesitebhisi yama-dentin anamaminerali nangenawo amaminerali ekugcineni kwe-apical.I-GBHA yembule ukusabela okumaphakathi kwe-Ca ku-matrix ye-dentin ye-circumpulpal engaxutshwe namaminerali lapho imakroskopu ye-electron yembule ukusatshalaliswa okucebile kwezinto ezinhle ezifana ne-electron-dense.I-matrix ye-dentin ye-mantle engenamaminerali yayingeyinhle ku-Ca kodwa iqukethe ama-matrix vesicles (MVs) amaningi agcwele i-crystalline kanye/noma idiphozi yamaminerali amorphous.Ukwenziwa kwamaminerali kwe-circumpulpal dentin kwenzeka ngaphandle kongqimba lwe-dentin ye-MV-rich mantle kuma-specimens athintekile.Idatha yethu ihlinzeka ngobufakazi be-histochemical bendawo engaba khona yokubophezela i-Ca ye-circumpulpal dentin matrix kanye nokungabikho kwayo ku-dentin ye-mantle lapho kwenzeka khona ukumbiwa kwe-MV-mediated.Ku-dentin ye-mantle, i-HEBP ayiphazamisi ukukhula kwekristalu kuma-MVs kodwa ivimbela ukuphuma kwayo ngemva kokuphuka kolwelwesi.Kuphakanyiswa ukuthi i-circumpulpal dentin matrix inamandla okwenza amaminerali ngaphandle kwe-MV-mediated mineralization ye-mantle dentin, nakuba ama-MV enquma indawo yokuqala kanye nesikhathi sokuchithwa kwe-dentin.