I-Penicillin G potassium usawoti CAS:113-98-4 99% Impuphu emhlophe
| Inombolo Yekhathalogi | XD90351 |
| Igama Lomkhiqizo | I-Penicillin G usawoti we-potassium |
| I-CAS | 113-98-4 |
| I-Molecular Formula | I-C16H17KN2O4S |
| Isisindo samangqamuzana | 372.48 |
| Imininingwane Yesitoreji | I-ambient |
| Ikhodi Yentela Evumelanisiwe | 29411000 |
Ukucaciswa Komkhiqizo
| Ukulahlekelwa Ekumisweni | <1.0% |
| Umbala Wesixazululo | Ayinawo umbala ogqamile kunokuqina okungu-Y2 noma i-YG2 kobubanzi bereferensi |
| pH | 5-7.5 |
| Izinto Ezihlobene | <1.0% |
| Isivivinyo | 99% |
| Amandla | 1440 - 1680u/mg |
| Ukubukeka | Impushana ecwebezelayo emhlophe ukuya cishe emhlophe |
| Ukucaca Kwesixazululo | Akukho ngaphezu kwe-opalescent kunokumiswa kwereferensi 1 |
| Ukudlulisa | I-NLT 90% |
| I-Butyl acetate | I-NMT 0.05% |
| I-Butanol | I-NMT 0.12% |
e wachaza ngaphambilini ukuthi i-molecule ye-autoinducer, ekhonjwe njenge-1,3-diaminopropane (1,3-DAP), ikhishwa i-Penicillium chrysogenum ne-Acremonium chrysogenum.Ukusebenzisa amasiko e-fermentor alawulwa yi-pH siye sabona kulo msebenzi ukuthi i-1,3-DAP ne-spermidine ivuselela ngokucacile i-biosynthesis ye-benzylpenicillin ku-P. chrysogenum, kokubili emithonjeni yokukhiqiza ye-penicillin echazwe futhi eyinkimbinkimbi.Kokubili i-1,3-DAP kanye ne-spermidine, kodwa hhayi i-purescine (1,4-diaminobutane), ikhiqiza ukwanda okukhulu kumazinga okubhalwa kwezakhi zofuzo ze-penicillin biosynthetic pcbAB, pcbC kanye ne-penDE.Lawa ma-polyamine akuthinti ukuvezwa kofuzo lwe-pH-stress regulator pacC yomhlaba wonke, ngaleyo ndlela kungafaki ukuthi umphumela we-1,3-DAP ne-spermidine kungenxa yokuguqulwa kwendlela yokulawula i-pH.Ukuvezwa kwezakhi zofuzo ezintathu ze-penicillin biosynthetic kuncipha kakhulu ku-laeA-knock-down mutant ye-P. chrysogenum, ekhiqiza amazinga aphansi kakhulu e-benzylpenicillin.Kuyathakazelisa ukuthi i-1,3-DAP kanye ne-spermidine zibuyisela umphumela wokuguqulwa kwe-laeA, ukubuyisela ngokuphelele amazinga okukhiqizwa kwe-penicillin.Ngaphezu kwalokho, i-1,3-DAP kanye ne-spermidine kuthuthukise ukubonakaliswa kwe-laeA ohlotsheni lwabazali futhi kwabuyisela amazinga emibhalo ye-laeA ku-laeA knock-down mutant.Ihlanganiswe ndawonye le miphumela ibonisa ukuthi umphumela ovuselelayo wama-molecule e-inducer 1,3-DAP kanye ne-spermidine wenziwa, okungenani ngokwengxenye, ngokukhuthazwa kwenkulumo ye-laeA, isilawuli somhlaba wonke esisebenza nge-epigenetically ekuvezweni kwezakhi zofuzo ze-metabolite yesibili. ngokuhlelwa kabusha kwe-heterochromatin.
