Imikhiqizo

I-Galactosidase ibhekisela ekilasini lama-enzyme enza i-hydrolyze izinto eziqukethe izibopho ze-galactosidic, njenge-lactose (i-lactose iyi-disaccharide eyakhiwe ukufiphala kokuphelelwa amanzi emzimbeni kwe-molecule eyodwa ye-glucose kanye ne-molecule eyodwa ye-galactose).Ihlukaniswe ikakhulukazi i-α-galactosidase ne-β-galactosidase.I-α-Galactosidase igqugquzela i-hydrolysis yamabhondi e-α-galactosidase, engaguqula futhi ibolise izici eziphikisana nokudla okunomsoco i-α-galactosides ekudleni nasekudleni kwesoya, futhi ithuthukise okuqukethwe kwayo okunomsoco.Ngaphezu kwalokho, i-enzyme iphinde ibe nezinhlelo zokusebenza ezithile embonini yezemithi, e-thickener kanye nezimboni zephepha.I-β-Galactosidase ayisetshenziswa nje kuphela futhi kabanzi embonini yokudla, kodwa futhi idlala indima ebalulekile emikhakheni ye-biotechnology efana nobunjiniyela bezakhi zofuzo, ubunjiniyela be-enzyme, ubunjiniyela bamaprotheni, njll., futhi isiqalile ukusetshenziswa kakhulu kwezokwelapha. nezinye izinkambu.

I-α-galactosidase (α-galactosidase, α-gal, EC 3.2.1.22) iyi-exoglycosidase eyenza i-hydrolysis yamabhondi e-α-galactosidic, futhi yaziwa nangokuthi i-melibiase ngoba ingabola i-melibiose, eyenza i-hydroctolysis ye-galactosidic izibopho.Lesi sici sikwenza kube usizo ekuthuthukiseni nasekuqedeni izakhi ezilwa nezakhamzimba ekudleni nasekudleni kwesoya.Ngaphezu kwalokho, ingakwazi ukubona ukuguqulwa kweqembu legazi le-B→O emkhakheni wezokwelapha, ilungise uhlobo lwegazi lomhlaba wonke, futhi ibambe iqhaza elibalulekile ekwelapheni esikhundleni se-enzyme ye-Fabry disease.I-Alpha-galactosidase ingase futhi isebenze kuma-polysaccharides ayinkimbinkimbi, ama-glycoprotein nama-sphingolipids aqukethe amabhondi e-alpha-galactosidic.Amanye ama-enzyme e-α-galactosidase nawo anomphumela we-transgalactosylation lapho ukuhlushwa kwe-substrate kucebile kakhulu, okungasetshenziselwa ukuhlanganiswa kwe-oligosaccharides kanye nokulungiswa kwe-cyclodextrin derivatives.Ukuthuthukiswa kwe-neutrophilic noma i-pH-stable α-galactosidase kanye nokuseshwa kwama-microorganisms noma izitshalo ezinokukhiqizwa kwe-enzyme ephezulu sekuphenduke indawo yocwaningo eminyakeni yamuva nje.Ama-α-galactosidase amaningi asebenzisa amandla e-thermostable nawo kancane kancane ahehe ososayensi abaningi ngenxa yezakhiwo zabo ezikhethekile, belindele ukusebenzisa amandla abo okushisa ukuze babambe iqhaza elikhulu ekusetshenzisweni kwezimboni, kanye nokukhombisa ukusetshenziswa okubanzi emkhakheni wesayensi nobuchwepheshe nemithi.amathemba ohlelo lokusebenza.