PIPES Cas: 5625-37-6 White crystalline powder 99% ABTS DIAMMONIUM SALT ULTRA PURE IBANGA
| Inombolo Yekhathalogi | I-XD90117 |
| Igama Lomkhiqizo | PIPES (Piperazine-1,4-bis(2-ethanesulfonic acid)) |
| I-CAS | 5625-37-6 |
| I-Molecular Formula | I-C8H18N2O6S2 |
| Isisindo samangqamuzana | 302.37 |
| Imininingwane Yesitoreji | I-ambient |
| Ikhodi Yentela Evumelanisiwe | 2933599 |
Ukucaciswa Komkhiqizo
| Izinsimbi ezinzima | <5ppm |
| Ukulahlekelwa Ekumisweni | <1.0% |
| Ukuncibilika | Isixazululo esicacile, esingenambala (5% 1N NaOH) |
| Isivivinyo | 99 - 101% |
| Ukubukeka | I-crystalline powder emhlophe |
PIPES [i-piperazine-N,N′-bis(2-ethanesulfonic acid)] ivamise ukusetshenziswa njenge-ejenti ebhafa ku-biochemistry.Kuyi-ethanesulfonic acid buffer eyakhiwe ngu-Good et al.ngawo-1960.I-PIPES ine-pKa eduze ne-pH ye-physiological eyenza isebenziseke emsebenzini we-cell culture.Kubhalwe phansi ukuze kuncishiswe ukulahleka kwe-lipid lapho kubhafa i-glutaraldehyde histology ezicutshini zezitshalo nezilwane.I-ejenti egcina isiphazamiso ene-pKa eduze ne-pH yemvelo.
I-antimicrobial peptide magainin 2 yakha ama-pores kulwelwesi lwe-lipid futhi idala ukungena kwe-membrane kokuqukethwe kwamaselula.Nakuba lokhu kungena kuyimbangela eyinhloko yomsebenzi wawo wokubulala amagciwane, indlela yokwakheka kwezimbotshana ayiqondwa kahle.Ngakho-ke siphenye ngokuningiliziwe ukusebenzisana kwe-mainin 2 ne-lipid membranes kusetshenziswa ama-single giant unilamela vesicles (GUVs).Ukubophezela kwe-mainin 2 kulwelwesi lwe-lipid lwama-GUV kukhuphule uguquko oluyingxenye endaweni yolwelwesi, δ, olwalulingana nokugxilisana okungaphezulu kwe-maginin 2, X. Lokhu kubonisa ukuthi izinga elingaguquki le-mainin 2-liyenge amabili -inguquko yesimo ukusuka esimweni esingaguquki ukuya embotsheni yembotshana inyuke kakhulu ngokunyuka kuka-δ.Ukushuba kolwelwesi lwe-lipid olulandela ukulangazelela kwe-GUV kuphinde kwasebenza ukwakheka kwezimbotshana ezi-2.Ukuze kuvezwe indawo ye-mainin 2, ukusebenzisana kwe-carboxyfluorescein (CF) -okulebulwe i-carboxyfluorescein 2 (CF-magainin 2) ene-GUV eyodwa equkethe i-fluorescent probe encibilika emanzini, AF647, yaphenywa kusetshenziswa i-confocal microscopy.Uma kungekho ukungezwani ngenxa yesifiso, ngemva kokusebenzelana kwe-main 2 amandla e-fluorescence we-GUV rim ngenxa ye-CF-magainin 2 anda ngokushesha ukuze abe inani eliqinile, elihlala lingashintshi isikhathi eside, futhi ngamasekhondi angu-4-32 ngaphambili. ukuqala kokuvuza kwe-AF647 ukuqina kwerimu kwaqala ukwanda ngokushesha kuya kwelinye inani elizinzile.Ngokuphambene, lapho kukhona ukungezwani, akukho ukwanda kokuqina kwerimu ngaphambi nje kokuqala kokuvuza kubonwe.Le miphumela ibonisa ukuthi i-mainin 2 ayikwazi ukuthutha isuka ngaphandle iye ku-monolayer yangaphakathi kuze kube ngaphambi nje kokwakheka kwezimbotshana.Ngokusekelwe kule miphumela, siphetha ngokuthi imbotshana eyenziwe nge-mainin 2 iyimbotshana eseluliwe esenziwe yasebenza futhi ukunwebeka kwe-monolayer yangaphakathi kungamandla ayinhloko aqhuba ukwakheka kwezimbotshana.
