I-Proteinase K Cas: 39450-01-6 Impushana emhlophe
| Inombolo Yekhathalogi | I-XD90191 |
| Igama Lomkhiqizo | Amaprotheni K |
| I-CAS | 39450-01-6 |
| I-Molecular Formula | I-C29H27N2O12P |
| Isisindo samangqamuzana | 626.50 |
| Imininingwane Yesitoreji | -15 kuya ku-20 ° C |
| Ikhodi Yentela Evumelanisiwe | 35079090 |
Ukucaciswa Komkhiqizo
| Inombolo Yekhathalogi | I-XD90191 |
| Igama Lomkhiqizo | Amaprotheni K |
| I-CAS | 39450-01-6 |
| I-Molecular Formula | I-C29H27N2O12P |
| Isisindo samangqamuzana | 626.50 |
| Imininingwane Yesitoreji | -15 kuya ku-20 ° C |
| Ikhodi Yentela Evumelanisiwe | 35079090 |
Ezilwaneni eziningi ezinama-chromosome ocansi u-X kanye no-Y, izindlela ezibanzi ze-chromosome zisetshenziswa ukulinganisa isisho sofuzo esixhumene no-X kwabesilisa nabesifazane.Ku-fly Drosophila melanogaster, indlela yesinxephezelo somthamo ngokuvamile idlulela kuma-transgene axhunywe ku-X.Imigqa engaphezu kuka-70 eshintshashintshayo ye-blowfly yezimvu yase-Australia i-Lucilia cuprina yenziwe njengengxenye yomzamo wokuthuthukisa izinhlobo zabesilisa kuphela zohlelo lokulawula izakhi zalesi silwane esiwuhlupho esikhulu sezimvu.Yonke imigqa iphethe isakhi sophawu seprotheyini ye-fluorescent evezwe ngendlela efanele.Kuyo yonke imigqa exhunywe ku-X engu-12, izibungu zesifazane zibonisa ukukhanya okukhanyayo kunezibungu zesilisa, okuphakamisa ukuthi isakhi sofuzo somaka asinxeshezelwa.Lokhu kuqinisekiswe i-RT-PCR yobuningi emigqeni ekhethiwe.Ukuze sinqume ukuthi ingabe izakhi zofuzo ezixhumene no-X ziyanxeshezelwa yini umthamo, sihlukanise izakhi zofuzo ezingu-8 ezingama-ortholog of genes aku-chromosome yesine ku-D. melanogaster.Ubufakazi bakamuva buphakamisa ukuthi i-D. melanogaster chromosome yesine, noma i-Muller element F, iyi-X chromosome yokhokho ku-Diptera ebuyele ku-autosome ohlotsheni lwe-Drosophila.Sibonisa nge-PCR yobuningi be-DNA yowesilisa nowesifazane ukuthi ufuzo lweqembu elingu-F elingu-6 lokuxhumanisa luhlala ku-X chromosome ku-L. cuprina.Ezinye izinhlobo ezimbili zofuzo ze-Muller element F zitholwe ziyi-autosomal ku-L. cuprina, kanti izinhlobo ezimbili zofuzo ze-Muller element B zitholwe endaweni efanayo ye-X chromosome njenge-L. cuprina orthologs ye-D. melanogaster Ephrin kanye nezakhi zofuzo ze-gawky.Sithola ukuthi izakhi zofuzo ze-chromosome ye-L. cuprina X zivezwa ngokulinganayo kwabesilisa nabesifazane (okungukuthi, umthamo unxeshezelwe ngokugcwele).Ngakho-ke, ngokungafani ne-Drosophila, kubonakala sengathi uhlelo lwesinxephezelo somthamo we-Lucilia luqondene ngqo nezakhi zofuzo ze-X chromosome futhi azikwazi ukuhlanganiswa ama-transgene asanda kufika.
