I-PUROMYCIN DIHYDROCHLORIDE Cas: 58-58-2 98% Impushana emhlophe
Inombolo Yekhathalogi | I-XD90195 |
Igama Lomkhiqizo | I-Puromycin dihydrochloride |
I-CAS | 58-58-2 |
I-Molecular Formula | I-C22H31Cl2N7O5 |
Isisindo samangqamuzana | 544.431 |
Imininingwane Yesitoreji | -15 kuya ku-20 ° C |
Ikhodi Yentela Evumelanisiwe | 29419000 |
Ukuhlukaniswa kwama-fibroblasts e-orbital abe ama-adipocyte avuthiwe kanye nokunqwabelana okulandelayo kwezicubu ze-adipose kukhonjisiwe ekuqhubekeleni phambili kwe-Graves' orbitopathy (GO).I-Autophagy ihileleke ku-adipogenesis, kodwa kuncane okwaziwayo ngendima ye-autophagy ekuqalisweni nasekuqhubekeleni phambili kwe-GO.Inhloso yalolu cwaningo ukuphenya indima ye-autophagy ku-pathogenesis ye-GO.Orbital adipose/connective tissue explants evela ezigulini ezine-GO kanye nezihloko ezivamile, kanye nama-fibroblasts ahlukanisiwe e-orbital, kwahlaziywa.I-Adipogenesis yenziwa kusetshenziswa indlela ehlukanisayo ene-hydrogen peroxide noma ngaphandle kwayo, futhi i-autophagy yashintshwa kusetshenziswa i-bafilomycin A1 ne-Atg5-targeted short hairpin RNA (shRNA).Ama-autophagosomes abonwa nge-electron microscopy.Ukuvezwa kofuzo oluhlobene ne-autophagy kanye nezici zokuloba ezihlobene ne-adipogenesis kwahlaziywa ngokusabela kweketana le-real reverse transcription-polymerase kanye/noma ukuhlaziywa kwe-Western blot.Ukunqwabelana kwamaconsi e-Lipid kwahlolwa nge-Oil Red O staining. Ama-vacuoles azenzakalelayo ayemaningi kakhulu kumaseli e-GO kunamaseli angewona awe-GO (p<0.05).Ukuvezwa kwezakhi zofuzo ezihlobene ne-autophagy bekuphakeme kakhulu kumathishu namaseli e-GO kunakuzakwabo abangewona ama-GO, ngokulandelana.I-Interleukin-1β ikhulise i-LC3-II, i-p62, ne-Atg7 amaprotheni kumaseli e-GO.Ukuqoqwa kwe-autophagosome kwaboniswa ngosuku lwesi-4 lwe-adipogenesis futhi kwehle ngosuku lwe-10, kanye nokwakheka kwamaconsi e-lipid.Ukuvezwa kwamaprotheni e-LC3 kanye ne-p62 kukhuphuke phakathi kwamahora angu-48 okuhlukaniswa futhi kwancipha kancane kancane kusukela osukwini lwesi-4 kuya ku-10. Ukwelashwa kwe-Bafilomycin A1 kanye ne-Atg5 knockdown nge-shRNA inhibited lipid droplet droplet kanye nokuvezwa okucindezelwe kwezimpawu ze-adipogenic.I-autophagy yanda kuzicubu ze-GO namaseli uma kuqhathaniswa namaseli e-GO izicubu namaseli okungewona awe-GO, okuphakamisa ukuthi i-autophagy idlala indima ku-GO pathogenesis.Ukukhohlisa kwe-Autophagy kungase kube okuhlosiwe kokwelapha kwe-GO.
I-4-Nitrophenyl-N-acetyl-β- D-glucosaminide iyi-substrate ewusizo yokuhlolwa kwe-colorimetric okusheshayo komsebenzi we-N-acetyl-b-glucosaminidase kumchamo womuntu.I-Chromogenic β-Glucosaminidase substrate ikhiqiza isixazululo esiphuzi lapho iqhekeka, esetshenziswa ikakhulukazi kumvubelo nezibundi.