Imikhiqizo

I-microRNA (miRNA) biosensor ezwela kakhulu esebenzisa i-ruthenium oxide nanoparticle (RuO(2) NP) -iqalwe ukwenziwa kwe-polymerization engu-3,3'-dimethoxybenzidine (DB) kanye nokubekwa kwesifanekiso se-miRNA se-insulating poly(3,3'-dimethoxybenzidine) (PDB) ifilimu ichazwe kulo msebenzi.I-biosensor yenziwe nge-monolayer exubile ye-oligonucleotide capture probes (CPs) kanye ne-4-mercaptoaniline ku-electrode yegolide.Kulandela ukuxutshwa nge-RuO(2) NP-tagged miRNA target, ingxube ye-DB/H(2)O(2) ku-pH 5.0 0.10 M i-acetate buffer isetshenziswe ku-biosensor.I-RuO(2) NPs isebenza njenge-polymerization initiator/catalyst ye-polymerization ye-DB.Futhi imicu ye-anionic miRNA ehlanganisiwe nama-CPs amahhala asebenza njengezifanekiso, eziqondisa ukubekwa kwe-PDB.Inani le-PDB efakiwe kanye namandla ayo okuvikela ahlotshaniswa ngokuqondile nokugxilisana kwe-miRNA eqondiwe esixazululweni.Ukuhlolwa kwe-electrochemical impedance spectroscopic kubonise ukuthi ubudlelwano bomugqa wokushaja-ukudluliselwa kokumelana nokugxilisa kusuka ku-6 .0 fM ukuya ku-2.0 pM bufinyelelwe ngemva kwamaminithi angu-60 wokufukamela kungxube ye-DB/H(2)O(2).Kwakungekho ukuhlanganisa phakathi kwe-pre-miRNA kanye ne-miRNA evuthiwe kanye nokuhlanganisa okuncane kakhulu phakathi kwamalungu omndeni we-miRNA ahlobene eduze ngisho nakuleveli yesisekelo esisodwa esingafani.Le biosensor esekwe kwi-impedance inikeza enye indlela ekhangayo yephrofayili yesisho se-miRNA futhi ingase inikeze amandla ukuthuthukiswa kwesistimu ephathekayo ye-multiplexing ye-miRNA yephrofayela.