I-TMB Cas:54827-17-7 99% Emhlophe, emhlophe ukuya kompunga noma impushana ephuzi
Inombolo Yekhathalogi | XD90163 |
Igama Lomkhiqizo | TMB |
I-CAS | 54827-17-7 |
I-Molecular Formula | I-C16H20N2 |
Isisindo samangqamuzana | 240.34 |
Imininingwane Yesitoreji | 2 kuya ku-8 °C |
Ikhodi Yentela Evumelanisiwe | 29215990 |
Ukucaciswa Komkhiqizo
Ukubukeka | Okumhlophe, okumhlophe kuya kokumpunga noma okuphuzi |
U-Asay | 99% |
Ukulahlekelwa Ekumisweni | <2.0% |
Okokusetshenziswa kocwaningo kuphela, hhayi okomuntu | ukusetshenziswa kocwaningo kuphela, hhayi ukusetshenziswa komuntu |
Izakhiwo: 3,3',5,5'-Tetramethylbenzidine i-white crystalline powder, ayinaphunga, ayinambitheki, ayincibiliki emanzini, incibilika kalula ku-acetone, i-ether, i-dimethyl sulfoxide, i-dimethylformamide, njll. Izinyibilikisi ze-Organic.
Ukulungiselela: I-3,3,5,5-Tetramethylbenzidine iyi-reagent ebalulekile ye-chromogen.Ukusebenzisa i-2,6-dimethylaniline njengempahla eluhlaza, ngokuvula, ukuhlanganisa i-oxidative nokuhlanzwa, kutholakala i-3,3,5,5-tetramethylbenzidine ehlanzekile, futhi isivuno esiphelele sifinyelela ku-65%.
Umsebenzi webhayoloji: I-TMB (BMblue) iyi-chromogenic substrate ye-immunohistochemistry ne-ELISA.
Okusetshenziswayo: Lo mkhiqizo awuwufaki umdlavuza esikhundleni se-benzidine 1 (ukuhlolwa kwe-Ames ukuthi awunayo), kufanelekile njenge-peroxidase substrate yokuhlolwa kwe-immunosorbent exhunywe yi-enzyme.I-substrate ikhiqiza umkhiqizo wokugcina oluhlaza okwesibhakabhaka oncibilikayo ongafundwa nge-spectrophotometrically ku-370 noma 620-650 nm.Ukusabela kwe-TMB kungamiswa nge-2MH2SO4 (iphenduka ibe phuzi) futhi ifundwe ku-50 nm ku-4Chemicalbook.I-reagent ezwelayo neqondile yokuthola igazi, ukulinganisa i-hemoglobin, nokulinganisa i-peroxidase.
Ukusetshenziswa: I-reagent entsha nephephile ye-chromogen;I-TMB kancane kancane ithathe indawo ye-benzidine eqinile ye-carcinogen nezinye izinto eziphuma ku-benzidine ye-carcinogenic, futhi isetshenziswa ekuhlolweni komtholampilo, ukuhlolwa kwe-forensic, ukutholwa kobugebengu kanye nokuqapha indawo ezungezile kanye neminye imikhakha;ikakhulukazi Ekuhlolweni kwe-biochemical yomtholampilo, i-TMB, njenge-substrate entsha ye-peroxidase, isetshenziswe kabanzi ku-enzyme immunoassay (EIA) kanye ne-enzyme-linked immunosorbent assay (ELISA);isetshenziswa kakhulu kumaphrojekthi alandelayo: ukutholwa kwezigxivizo zeminwe yegazi;Ukutholwa ngokushesha kotshwala ematheni;ukulungiswa kwemichilo yokuhlola umchamo;ukutholakala kwegciwane le-hepatitis;ukuhlolwa kokutholwa kokukhulelwa;ukuzimisela okusheshayo kwe-glucose, i-hemoglobin, i-albhamuin egazini nomchamo;ukuhlolwa kwegazi okunendle;ukunqunywa kwenani le-granulocyte egazini, i-steroids, Ukutholwa kwamahomoni ocansi;ukunqunywa komsebenzi we-enzymatic;ukutholakala kwama-antigen, amasosha omzimba kanye nezinto zofuzo