I-Tris maleate Cas:72200-76-1 99.5% I-crystalline powder emhlophe
Inombolo Yekhathalogi | XD90057 |
Igama Lomkhiqizo | Tris maleate |
I-CAS | 72200-76-1 |
I-Molecular Formula | C4H11NO3 · C4H4O4 |
Isisindo samangqamuzana | 237.21 |
Imininingwane Yesitoreji | I-ambient |
Ikhodi Yentela Evumelanisiwe | 29221985 |
Ukucaciswa Komkhiqizo
Ukubukeka | I-crystalline powder emhlophe |
Isivivinyo | ≥ 99.5% |
I-Cocrystallization ye-lysyl-tRNA synthetase evela ku-Thermus thermophilus nama-tRNAly ayo ahlangene kanye ne-Escherichia coli tRNAlys.
I-Lysyl-tRNA synthetase evela ku-Thermus thermophilus ihlanganiswe ne-tRNAlys yayo ehlangene noma i-Escherichia coli tRNAlys kusetshenziswa i-ammonium sulfate njenge-precipitant.Amakristalu akhula esuka kuzixazululo eziqukethe i-1:2.5 stoichiometry ye-synthetase dimer ukuya ku-tRNA ngo-18-22% ammonium sulfate ku-50 mM Tris-maleate buffer ku-pH 7.5.Zombili lezi zakhiwo zakha amakristalu angama-square prismatic, ama-tetragonal anamapharamitha eyunithi yeyunithi afana kakhulu (a = b = 233 A, c = 119 A) futhi ahlukanise okungenani abe ngu-2.7 A ukulungiswa.Nokho i-homocomplex ingeyeqembu lesikhala i-P42(1)2 kanye ne-heterocomplex yeqembu lesikhala i-I422.