Amagciwane angamakholoni, aboniswa yi-Bacteroides thetaiotaomicron, adlala indima ebalulekile ekugcineni impilo yomuntu ngokuhlanganisa imindeni emikhulu yama-glycoside hydrolases (GHs) ukuze asebenzise ama-polysaccharides okudla futhi abambe ama-glycans njengezakhi.Ukwandiswa komndeni we-GH okunjalo kuboniswa umndeni we-23 we-GH92 glycosidase ofakwe nge-B. thetaiotaomicron genome.Lapha sibonisa ukuthi lawa angama-alpha-mannosidase asebenza ngendlela eyodwa yokususa ukuze asebenzise i-N-glycans engusokhaya.Isakhiwo esinezinhlangothi ezintathu se-GH92 mannosidase sichaza umndeni wamaphrotheni anezizinda ezimbili lapho isikhungo se-catalytic sitholakala ku-interface yesizinda, sihlinzeka ngosizo lwe-asidi (glutamate) nesisekelo (aspartate) ku-hydrolysis ku-Ca(2+) - indlela encike.Izakhiwo ezinezinhlangothi ezintathu ze-GH92 eziyinkimbinkimbi ezinama-inhibitors zinikeza ukuqonda mayelana nokucaciswa, indlela kanye nohambo oluhambisanayo lwe-catalysis.I-Ca(2+) idlala indima ebalulekile ekusizeni ukuhlanekezela i-mannoside kude ne-ground-state yayo (4)C(1) ukuhambisana kwesitulo kuya kusimo soguquko.
