Inoveli yofuzo lwe-β-glucosidase, bgl1G5, yenziwe yavela kuPhialophora sp.G5 futhi ivezwe ngempumelelo ku-Pichia pastoris.Ukuhlaziywa kokulandelana kubonise ukuthi isakhi sofuzo siqukethe uhlaka lokufunda oluvulekile lwe-1,431-bp olufaka ikhodi yephrotheni engama-amino acid angama-476.Ukulandelana kwe-amino acid etholiwe ye-bgl1G5 kubonise ubunikazi obuphezulu obungu-85 % obunophawu lwe-β-glucosidase evela ku-Humicola grisea yomndeni we-glycoside hydrolase 1. Uma kuqhathaniswa nabanye ozakwabo besikhunta, i-Bgl1G5 ibonise umsebenzi ofanayo ophezulu ku-pH 6.0 kanye ne-50 °C ku-pH 5.0–9.0.Ngaphezu kwalokho, i-Bgl1G5 ibonise ukushiseka okuhle kokungu-50 °C (amahora angu-6 uhhafu wempilo) nomsebenzi othize ophezulu (54.9 U mg–1).Amanani e-K m kanye ne-V max abheke ku-p-nitrophenyl β-D-glucopyranoside (pNPG) abengu-0.33 mM no-103.1 μmol min–1 mg–1, ngokulandelana.Ukuhlolwa kokucaciswa kwe-substrate kubonise ukuthi i-Bgl1G5 yayisebenza kakhulu ngokumelene ne-pNPG, ibuthakathaka ku-p-nitrophenyl β-D-cellobioside (pNPC) kanye ne-p-nitrophenyl-β-D-galactopyranoside (ONPG), futhi yayingenawo umsebenzi ku-cellobiose.
