Isakhi sofuzo se-beta-glucosidase (bgl3) esivela ku-Streptomyces sp.I-QM-B814 (Iqoqo Le-American Type Culture 11238) yenziwe yahlanganiswa ngokuhambisana nokusebenza kwe-beta-glucosidase-negative mutant ye-Streptomyces lividans.Uhlaka lokufunda oluvulekile lwama-nucleotide angu-1440 olufaka i-polypeptide yama-amino acid angu-479 lutholwe ngokulandelanisa.Iphrotheni efakwe ikhodi (Bgl3) ikhombisa ukufana okubanzi (ngaphezu kuka-45% ubunikazi) nama-beta-glycosidase avela kumndeni-1 glycosyl hydrolases.I-enzyme ehlanganisiwe, ehlanjululwe ngokulandela imvula ye-ammonium sulphate kanye nezinyathelo ezimbili ze-chromatographic, i-monomeric enesisindo samangqamuzana angu-52.6 kDa, njengoba kunqunywa i-mass spectrometry, kanye nephoyinti le-isoelectric le-pI 4.4.I-enzyme ibonakala iyi-beta-glucosidase enokucaciswa okubanzi kwe-substrate, iyasebenza kuma-cellooligomers, futhi yenza ukusabela kwe-transglycosylation.Amanani alinganiselwe we-Km e-p-nitrophenyl-beta-D-glucopyranoside kanye ne-cellobiose angu-0.27 mM no-7.9 mM, ngokulandelanayo.Amanani e-Ki eglucose ne-delta-gluconolactone, kusetshenziswa i-p-nitrophenyl-beta-D-glucopyranoside njenge-substrate, angu-65 mM no-0.08 mM, ngokulandelana.I-enzyme ecwengiwe ine-pH elungile engu-pH 6.5 futhi izinga lokushisa eliphakeme lomsebenzi ngu-50 degrees
