I-6-Chloropurine CAS:87-42-3 Impushana ephuzi ekhanyayo
| Inombolo Yekhathalogi | XD90547 |
| Igama Lomkhiqizo | 6-Chloropurine |
| I-CAS | 87-42-3 |
| I-Molecular Formula | I-C5H3ClN4 |
| Isisindo samangqamuzana | 154.557 |
| Imininingwane Yesitoreji | 2 kuya ku-8 °C |
| Ikhodi Yentela Evumelanisiwe | 2933990090 |
Ukucaciswa Komkhiqizo
| Ukubukeka | Impushana ephuzi ekhanyayo |
| Isivivinyo | 99% |
Sisanda kubika ukuthi i-pyrene nucleotide ifakwa ngokukhethekile ngokuphambene nendawo ye-abasic, i-3'-T ye-thymine dimer, kanye nezisekelo ezingonakalisiwe kakhulu yi-yeast DNA polymerase eta (pol eta).Ngenxa yokuthi i-pyrene iyi-molecule engelona i-polar engenakho i-H-bonding, ukusebenza kahle okuphezulu ngokungavamile kokufakwa kwe-dPMP kuhlotshaniswa nekhono layo eliphakeme lokupakisha isisekelo, futhi kugcizelela ukubaluleka kokunqwabelanisa kwesisekelo ekukhethweni kwama-nucleotide yi-pol eta.Ukuze siphenye indima ye-H-bonding kanye ne-base pair geometry ekukhethweni kwama-nucleotide nge-pol eta, sinqume ukufaneleka kokufakwa kwama-nucleotides aguquliwe okuyisisekelo 2,6-diaminopurine, 2-aminopurine, 6-chloropurine, ne-inosine okungase yenza inombolo ehlukile yamabhondi e-H ngesisekelo sesifanekiso kuye nge-base pair geometry.Ukumataniswa kwesisekelo se-Watson-Crick kubonakala kudlala indima ebalulekile ekukhetheni ama-nucleotide analogue ukuze afakwe ngokuphambene no-C no-T njengoba kufakazelwa ukwehla kokusebenza kahle kokufakwa okuhlobene nokuncipha kwenani lamabhondi e-Watson-Crick H kanye nokwenyuka inani lokusebenzelana komnikeli nomamukeli nowamukelayo.Ukukhethwa kokufakwa kwe-nucleotide kukhulu ngokuphambene no-5'-T kuno-3'-T we-thymine dimer, ngokuvumelana nomsebenzi odlule ophakamisa ukuthi i-5'-T ibanjwe ngokuqinile kune-3'-T.Ngaphezu kwalokho, ukufakwa kuka-A okuphambene kokubili u-T we-dimer kubonakala kulamula ukumataniswa kwesisekelo kwe-Watson-Crick hhayi ukumataniswa kwesisekelo kwe-Hoogsteen okusekelwe ekufakeni okucishe kufane okufanayo kwe-A kanye ne-7-deaza-A, eyokugcina engenalo i-H- amandla okuxhumana ku-N7.Ukusebenza kahle okulinganiselwe kokufakwa kwama-nucleotide angakha amapheya esisekelo e-Watson-Crick afana nalawo ocezu lwe-Klenow, kuyilapho ucezu lwe-Klenow lubandlulula kakhulu ukungafani, ngokuhambisana nokukhetha komumo okukhulu.Le miphumela igcizelela ukubaluleka kwe-H-bonding ne-Watson-Crick base geometry ekukhethweni kwama-nucleotide yizo zombili i-pol eta kanye nesiqephu se-Klen Ow, kanye nendima encane yokukhetha umumo ekufakweni yi-pol eta ngenxa yokuvuleka nokuncane kwayo. isayithi elisebenzayo elivinjelwe.
