Imikhiqizo

Izendlalelo zebhulashi le-polymer ezisekelwe ku-block copolymers ye-poly(oligo(ethylene glycol) methacrylate) (POEGMA) kanye ne-poly(glycidyl methacrylate) (PGMA) zakhiwe kumawafa e-silicon yizicishazi ezikhiqizwe ukudluliswa kwe-athomu ye-electron radical polymerization (AGET ATRP).Izinhlobo ezahlukene ze-biomolecule zingahlanganiswa kulezi zingqimba zebhulashi ngokusabela kwamaqembu e-PGMA epoxide anamaqembu e-amino ku-biomolecule, kuyilapho i-POEGMA, emelana nokukhangiswa kwamaprotheni okungaqondile, ihlinzeka ngendawo evimbela ukungcola.Izinto ezingaphezulu bezibonakala nge-engeli yokuxhumana namanzi, i-ellipsometry, kanye ne-Fourier transform infrared spectroscopy (FTIR) ukuze kuqinisekiswe ukusabela kokulungiswa.Ukuhlukaniswa kwesigaba samabhulokhi we-copolymer ezendlalelo kwabonwa yi-AFM.Umthelela wezakhiwo ezingaphezulu ekuhlanganisweni kwamaprotheni waphenywa kusetshenziswa izindlela ze-radiolabeling.Kwaboniswa ukuthi izindawo ezingaphezulu ezinezendlalelo ze-POEGMA zazimelana namaprotheni, kuyilapho inani lamaprotheni elihlanganiswe endaweni eguquliwe ye-diblock copolymer likhuphuka ngokunyuka kogqinsi lwesendlalelo se-PGMA.Umsebenzi we-lysozyme ohlanganiswe phezulu nawo ungalawulwa ngokushintsha ukujiya kongqimba lwe-copolymer.Lapho i-biotin ihlanganiswa nokuxhunyelelwa kwe-block copolymer, indawo engaphezulu yahlala imelana ne-nonspecific protein adsorption kodwa yabonisa ukubopha okuqondile kwe-avidin.Lezi zakhiwo, okungukuthi, inani elilawulwa kahle kanye nomsebenzi wama-biomolecules ahlanganisiwe kanye nokucaciswa kokusebenzelana nama-biomolecules okuqondiwe kungase kusetshenziswe ukuze kuthuthukiswe isilinganiso sesignali-kumsindo ekusetshenzisweni kwezinzwa.Ngokuvamile, izindawo ezinjalo ezingaphezulu zingase zibe usizo njengezinto zokuqaphela izinto eziphilayo zokucaciswa okuphezulu kokusebenza kwe-biomaterials.