Imikhiqizo

Ukusebenza kwamangqamuzana amagciwane ekwelapheni i-Pseudomonas aeruginosa pneumonia kuyehla ngenxa yokusatshalaliswa kokumelana namagciwane.Ukuba khona kwezinhlelo zokuxhumana ngamabhaktheriya, njengokuzwa isibalo sekhoramu, kunikeze amathuba amasha okwelashwa.Ama-lactonase acisha ngempumelelo i-acyl-homoserine lactone-based bacterial quorum sensing, ebeka lawa ma-enzyme njengemithi emisha elwa ne-Pseudomonas engase ihlolwe inyumoniya.Inhloso yocwaningo lwamanje kwakuwukuhlola ikhono le-lactonase ebizwa ngokuthi i-SsoPox-I ukufa kwegundane P. aeruginosa pneumonia.Ukuhlola ukuqedwa kwekhoramu ye-SsoPox-I-mediated, siqale salinganisa umsebenzi we-virulence gene lasB, ukuhlanganiswa kwe-pyocianin, umsebenzi we-proteolytic wokumiswa kwebhaktheriya kanye nokwakheka kwe-biofilm ye-a. I-PAO1 uhlobo olukhule phambi kwe-lactonase.Kumodeli ebulalayo eyingozi ye-P. aeruginosa pneumonia kumagundane, sihlaziye imiphumela yokwelashwa kwangaphambi kwesikhathi noma okuhlehlisiwe ku-intracheal nge-SsoPox-I ekufeni, inani lebhaktheriya lamaphaphu kanye nokulimala kwamaphaphu. , i-pyocianin synthesis, umsebenzi we-proteolytic kanye nokwakheka kwe-biofilm.Ukusetshenziswa kusenesikhathi kwe-SsoPox-I kunciphisa ukufa kwamagundane ane-pneumonia eyingozi kusuka ku-75% kuya ku-20%.Umonakalo wamaphaphu we-histological wehliswe kakhulu kodwa isibalo sebhaktheriya samaphaphu asishintshwanga ngokwelashwa.Ukwelashwa okubambezelekile kwakuhlotshaniswa nokunciphisa okungabalulekile kokufa.Le miphumela ibonisa imiphumela yokuvikela ye-lactonase SsoPox-I ku-P. aeruginosa pneumonia futhi ivula indlela yokusetshenziswa kokwelapha kwesikhathi esizayo.