Imikhiqizo

1.Ukushintshashintsha komsebenzi wama-enzyme ahlotshaniswa ne-carbohydrate metabolism kubalulekile kumazambane abandayo-induced sweetening (CIS).Inoveli yofuzo lwe-RING finger SbRFP1 yenziwa i-clonic futhi ukubonakaliswa kwayo kwatholakala ukuthi akunamakhaza ezigabeni zamazambane ze-CIS-resistant genotypes.Ukuguqulwa kwe-SbRFP1 kumazambane kuqinisekisile indima yayo ekuvimbeleni i-β-amylase nomsebenzi we-invertase, okwathi ngenxa yalokho kwehlisa isivinini sokuwohloka kwesitashi kanye ne-sucrose kanye nokunqwabelana kokunciphisa ushukela ezigabeni ezigcinwe emakhazeni.Lokhu okutholakele kusikisela ngokuqinile ukuthi i-SbRFP1 ingasebenza njengomlawuli ongemuhle we-BAM1 kanye ne-StvacINV1 ukuze kwehliswe ukunqwabelana kokunciphisa ushukela ohlelweni lwe-CIS yamazambane.

2.Inhloso yalo msebenzi bekuwukufunda umthelela wokupheka isitimu ku-pectin methylesterase (PME) kanye nemisebenzi engapheli ye-α- kanye ne-β-amylase ezicutshini ezihlukene (i-cortex ne-pith) yamazambane aluhlaza naphethwe ngokushisa cv.Agria.Kukhethwe amazinga okushisa amathathu ahlukene okupheka (55, 70 kanye nama-85 °C).Esivivinyweni ngasinye sokupheka, amaphrofayli ezinga lokushisa lesikhathi arekhodwa kanye nezinga lokupheka lavezwa ngokwesici sokupheka.Ukupheka ngomusi kube nomthelela ekwenzeni kusebenze i-PME ku-55 °C kanye nokunciphisa umsebenzi wayo ezingeni lokushisa lokugcina (85 °C) , enenani eliphakeme kakhulu ku-cortex (0.3745 ± 0.0007 µmol galacturonic acid (GA) g(-1) isisindo esisha (FW) min(-1) ) uma kuqhathaniswa ne-pith (0.2617 ± 0.0012 µmol GA g(-1) FW iminithi(-1) ).Ukuba khona kwama-isoforms ane-heat-labile kanye nezinzile ekushiseni kwe-PME kumathishu amazambane acatshangwayo nakho kwacatshangelwa.Ukwelashwa kokushisa ngesitimu kuholele ekwehleni okukhulu kwemisebenzi ye-endo native α- kanye ne-β-amylase kuzo zombili izicubu uma kuqhathaniswa nezambane elingavuthiwe, nakuba ngaphandle kokuvalwa ngokuphelele.Ama-enzyme ehlisa isitashi nawo atholakala esakazwa ngendlela ehlukile ku-tuber eluhlaza.Ukupheka kwe-steam kuthinteke ngezindlela ezihlukene umsebenzi wensalela we-enzymatic ohloliwe ezicutshini ezicatshangelwayo zamazambane cv.Agria.Ucwaningo olwengeziwe luyadingeka ukuze kuqinisekiswe imiphumela etholiwe.