Imikhiqizo

Isingeniso: I-BSA ingelinye lamaprotheni asetshenziswa kakhulu ezindaweni zokusebenzela zamakhemikhali ezinto eziphilayo, futhi ukubaluleka kwayo kungase kunganakwa ekuhlolweni ngenxa yokuthi ivame kakhulu futhi ivamile kakhulu.I-Bovine serum albumin (BSA), eyaziwa nangokuthi ingxenye yesihlanu, iyi-globulin ku-bovine serum equkethe izinsalela ze-amino acid ezingama-583, enesisindo samangqamuzana esingu-66.430kDa kanye nephuzu le-isoelectric elingu-4.7.I-BSA inohlu olubanzi lwezinhlelo zokusebenza ezivivinyweni ze-biochemical, njenge-ejenti evimbelayo kuma-western blots.

Isicelo: I-Bovine Serum Albumin (BSA), eyaziwa nangokuthi ingxenye yesihlanu, iyi-globulin ku-serum yezinkomo, equkethe izinsalela ze-amino acid ezingu-607, futhi inohlu olubanzi lwezinhlelo zokusebenza ekuhloleni kwamakhemikhali ezinto eziphilayo.I-albumin ye-bovine serum ngokuvamile isetshenziswa njengesizinzisi kusixazululo sesitoreji nesisombululo sokusabela se-enzyme evimbela noma ama-enzyme aguquliwe ukuze kunzinziswe umsebenzi we-enzyme futhi kuvimbele ukubola kwe-enzyme kanye nokukhangiswa okungaqondile.

Umsebenzi: I-BSA ivamise ukusetshenziswa njengesizinzisi kusixazululo sesitoreji kanye nesisombululo sokusabela se-enzyme evimbela noma ama-enzyme aguquliwe, ngoba amanye ama-enzyme awazinzile noma anomsebenzi ophansi ekugxilweni okuphansi.Ngemva kokwengeza i-BSA, ingase idlale indima "yokuvikela" noma "umthwali", futhi umsebenzi wama-enzyme amaningi ungathuthukiswa kakhulu ngemva kokwengeza i-BSA.Ama-Enzymes angadingi ukwengezwa kwe-BSA ngokuvamile awathintwa ukwengezwa kwe-BSA.Eningini le-DNA engaphansi, i-BSCA ingenza ukugaya kuphelele, futhi ingafinyelela ukusika okuphindaphindiwe.Ku-37°C, lapho ukusabela kokugaya kudlula ihora elingu-1, i-BSA ingenza i-enzyme izinze, ngoba ku-reaction buffer ngaphandle kwe-BSA, ama-enzyme amaningi avimbela angaphila kuphela ku-37°C imizuzu engu-10~20 noma ngisho nesikhathi esifushane..Ngokuphambene, i-BSA ingabopha ama-ion ensimbi namanye amakhemikhali ku-buffer noma i-substrate DNA evimbela umsebenzi wokukhawulelwa kwama-endonuclease.

Ukusetshenziswa: I-albumin ye-bovine serum (i-BSA, i-StandardGrade) yezinga elijwayelekile, engahlangabezana nezidingo zokuhlolwa okuningi okuyinjwayelo, okufana ne-ejenti evimba amasosha omzimba, iseli lezicubu (amangqamuzana ezilwane ezincanyana namaseli ezinambuzane, njll.) izakhi zesiko kanye nezingxenye zesiko, ukuqiniswa kwamaprotheni/i-enzyme ama-reagents kanye namazinga we-protein quantification.I-albumin ye-bovine serum yezinga lokuxilonga (i-BSA, i-DiagnosticGrade) ingahlangabezana nezidingo eziningi zokuhlola ezijwayelekile, njenge-ejenti evimba umzimba, i-protein/enzyme stabilizer, i-diluent, carrier kanye nezinga lamaphrotheni.Ngaphezu kwalokho, ingase isetshenziselwe ukuhlola ama-immunoassays, isiko lamaseli kanye nokuhlolwa kwe-hybridization okudinga ukuzwela okukhulu.