I-Cytidine-5'-triphosphate (CTP), usawoti we-disodium CAS:652154-13-7
| Inombolo Yekhathalogi | XD90551 |
| Igama Lomkhiqizo | I-Cytidine-5'-triphosphate (CTP), usawoti we-disodium |
| I-CAS | 652154-13-7 |
| I-Molecular Formula | I-C9H14N3Na2O14P3 |
| Isisindo samangqamuzana | 527.12 |
| Imininingwane Yesitoreji | 2 kuya ku-8 °C |
Ukucaciswa Komkhiqizo
| Ukubukeka | Impushana emhlophe |
| Isivivinyo | 99% |
I-CTP:phosphocholine cytidylyltransferase α (CCTα) iyi-enzyme yenuzi eyenza isinyathelo sokunciphisa izinga ku-CDP-choline pathway for phosphatidylcholine (PC) synthesis.Ukusebenza kwe-Lipid kwe-CCTα kuphumela ekushintsheni kwayo emvilophini yenuzi kanye nokwandiswa kwenethiwekhi ye-intranuclear membrane ebizwa ngokuthi i-nucleoplasmic reticulum (NR) ngomshini ohilela ukuguqulwa kolwelwesi.Amalambu enuzi nawo ayadingeka ukuze kuzinze kanye nokwanda kwe-NR, kodwa ukuthi lesi sakhiwo esiyingqayizivele, noma i-lamina yenuzi ngokuvamile, iyadingeka ekuhlanganiseni kwe-PC akwaziwa.Ukuhlola lobu budlelwano, i-nuclear lamina yaqedwa yi-RNAi noma yaphazanyiswa ukubonakaliswa kwe-Hutchinson-Gilford progeria syndrome (HGPS) i-mutant lamin A (progerin), futhi umphumela ku-CCTα kanye ne-choline metabolism yahlaziywa.Ukuthuliswa okuphakathi kwe-siRNA kwe-lamin A/C noma i-lamin B1 kumaseli e-CHO ukuze kwehliswe i-NR akuzange kube nomthelela ekuhlanganiseni kwe-PC, kuyilapho ukugoqa okuphindwe kabili kungavimbeli ngokuqondile indlela.Ukuqinisekisa t indima yakhe encane ekuhlanganiseni kwe-PC, yi-10% kuphela ye-choline/ethanolamine phosphotransferase eyeqile okwesikhashana etholwe ku-NR.Kumaseli e-CHO, i-CCTα yayiyi-nucleoplasmic futhi ihlanganiswe ne-GFP-progerin kumafolda enuzi kanye nokuhlasela;noma kunjalo, ama-fibroblasts e-HGPS abonise ukusatshalaliswa okungavamile kwe-CCTα ku-cytoplasm kanye nemvilophu yenuzi ehambisana nokuncishiswa okuphindwe kabili kwe-PC synthesis.Naphezu kokushintsha kwendawo yayo, ukuhlolwa kokulebula kwe-choline kubonise ukuthi umsebenzi we-CCT wawungathinteki, futhi ukuvinjelwa kwe-PC synthesis kwalandelwa umsebenzi oncishisiwe we-hemicholinium-sensitive choline transporter.Siphetha ngokuthi i-CCTα nama-lamin asebenzisana ngokukhethekile ukuze akhe i-NR, kodwa ukwakheka okuphelele kwemvilophu yenuzi kunomthelela omncane emsebenzini we-CCT kanye nokuhlanganiswa kwe-PC.
