Imikhiqizo

I-Tet 5-methylcytosine dioxygenase yenza i-DNA demethylation ikhiqize i-5-hydroxymethylcytosine neminye imikhiqizo ene-oxidized.I-Tet1 ne-Tet2 zivezwa kakhulu kumaseli e-pluripotent egundane futhi ehliselwe ezingeni elihlukile kumaseli e-somatic, kodwa izindlela zokuloba azicacile.Lapha sichaze izizinda zomgqugquzeli kanye nezithuthukisi ku-Tet1 ne-Tet2.Ngaphakathi kwe-"superenhancer" engu-15-kb ye-Tet1, kukhona amasayithi amabili okuqala okulotshiweyo (ama-TSS) anamaphethini okwenza kusebenze ahlukene phakathi nokuthuthukiswa.Isifunda somgqugquzeli we-6-kb phezulu nomfula we-distal TSS sisebenza kakhulu kumaseli angenangqondo we-pluripotent, sibika ngokuzenzakalelayo isisho se-Tet1 kusistimu ye-transgenic, futhi sithola ngokushesha i-DNA methylation nokuthuliswa lapho kuhlukaniswa amaseli akhulisiwe kanye ne-epiblast yomdabu.I-TSS yesibili ezansi nomfula, ehlotshaniswa nomgqugquzeli ocebile we-CpG obuthakathaka, yenziwa yasebenza isithuthukisi esingumakhelwane kumaseli we-embryonic stem cell (ESCs) namaseli afana ne-epiblast (EpiLCs).I-Tet2 ine-CpG island p romoter enomsebenzi ozimele we-pluripotency kanye nesithuthukisi esikhethekile se-ESC se-distal intragenic;lokhu kokugcina kwehliswa ngokushesha kuma-EpiLC.Ucwaningo lwethu lwembula izindlela ezihlukile zokulawulwa kokulotshiweyo ku-Tet1 ne-Tet2 phakathi noshintsho lwesimo seseli lokuthuthuka kwangaphambi kwesikhathi.Izintatheli ezintsha ze-transgenic ezisebenzisa izizinda zokulawula i-Tet1 ne-Tet2 zingase zisebenze ukuhlukanisa izinguquko eziguquguqukayo ezifundeni ze-pluripotent kanye nokuhluka okuyisisekelo kwe-epigenetic.