Imikhiqizo

I-Adenosine iyisilawuli esingapheli sethoni ye-vascular.Lo msebenzi we-adenosine unqanyulwa ngokuthathwa kwayo kanye ne-metabolism ngamangqamuzana e-microvascular endothelial (MVEC).Isithuthi esihamba phambili esihilelekile yi-ENT1 (i-equilibrative nucleoside transporter subtype 1).I-MVEC iphinde iveze i-nucleobase transporter (ENBT1) ehilelekile ku-flux yeselula ye-adenosine metabolites njenge-hypoxanthine.Izinguquko kunoma iyiphi yalezi zinhlelo zokuthutha zingaba nomthelela ekusebenzeni kwe-adenosine kanye nemetabolism yayo, okuhlanganisa ukwakheka kwama-radicals angenawo umoya-mpilo.I-MVEC ehlukanisiwe nemisipha yohlaka lwamathambo e-ENT1(+/+) kanye ne-ENT1(-/-) amagundane aye ngaphansi kwengcindezi ye-oxidative edalwe ukulingisa ischemia/reperfusion noma i-menadione.Imisebenzi yokusebenza ye-ENT1 kanye ne-ENBT1 iye yahlolwa ngokusekelwe ku-zero-trans influx kinetics yama-substrates abhalwe nge-radiolabele.Kube nokuncishiswa kwezinga lokutholwa kwe-ENBT1-mediated hypoxanthine yi-ENT1(+/+) MVEC ephathwa nge-menadione noma ngemva kokuchayeka ezimeni ezilingisa isc hemia/reperfusion.Kuzo zombili izimo, i-superoxide dismutase mimetic MnTMPyP yehlise ukulahleka komsebenzi we-ENBT1, ifaka ama-superoxide radicals empendulweni.Ngokuphambene, i-MVEC ekhishwe yodwa kusukela ku-ENT1(-/-) amagundane abonisanga ukuncipha komsebenzi we-ENBT1 ekwelapheni nge-menadione noma ukulingisa ischemia/reperfusion, kodwa abe nezinga eliphezulu kakhulu lomsebenzi we-catalase ngokuhlobene ne-ENT1(+/+) MVEC.Le datha iphakamisa ukuthi umsebenzi we-ENBT1 wehlile ku-MVEC ngenxa yokukhuphuka kwe-superoxide radical ehlotshaniswa nokulimala kwe-ischemia/reperfusion.I-MVEC ihlukanisiwe kumagundane e-ENT1(-/-) awakubonisi lokhu kwehliswa ku-ENBT1, okungenzeka ngenxa yokwanda komsebenzi we-catalase.