Imikhiqizo

Ukuphenya imiphumela ye-extranase yangaphandle ne-sodium fluoride ku-S. mutans monospecies biofilm.S.mutans 25175 yakhuliswa ku-tryptone soya broth medium, futhi i-biofilm yakhiwa kumaslayidi engilazi ane-1.0% sucrose.I-dextranase yangaphandle ne-sodium fluoride yengezwe iyodwa noma ndawonye.I-biofilm morphology yahlaziywa nge-confocal laser scanning microscopy.Imiphumela yomuthi ekunamatheleni nasekukhiqizweni kwe-exopolysaccharide ngama-biofilms yahlolwa ngokubala kwe-scintillation kanye nendlela ye-anthrone, ngokulandelana.Kulolu cwaningo, sithole ukuthi ukwakheka kwe-biofilm yokuqala kanye ne-biofilm evuthiwe yashintshwa ngokwengxenye yi-dextranase kanye nokugxila okuphezulu kwe-biofilm. i-sodium fluoride ngokwehlukana.Kodwa-ke, i-dextranase ehlangene ne-concentration ephansi ye-sodium fluoride ingabhubhisa ngokucacile isakhiwo esifana nesihlahla se-biofilm, futhi iholele ekunamatheleni okuncane kwebhaktheriya kunalapho i-dextranase noma i-fluoride isetshenziswa iyodwa (P <0.05).Amanani e-exopolysacchari de ancibilikayo futhi angancibiliki ancishiswa kakhulu ngokuhlanganisa i-dextranase ne-concentration ephansi ye-sodium fluoride, kakhulu kunalokho lapho esetshenziswa yedwa (P <0.05).Le datha ibonisa ukuthi i-dextranase kanye nokuhlushwa okuphansi kwe-sodium fluoride kungase kube nemiphumela ye-synergistic ngokumelene ne-S. mutans biofilm futhi iphakamisa ukusetshenziswa kokuhlushwa okuphansi kwe-sodium fluoride ekwelashweni kwe-anticaries.