Imikhiqizo

I-3-Morpholinepropanesulfonic acid iyisigcinalwazi sebhayoloji futhi ivamise ukusetshenziselwa ukulungisa amabhafa we-RNA electrophoresis.

Izimo zesitoreji:I-3-Morpholinepropanesulfonic acid iyi-asidi ye-sulfonic, futhi kufanele igcinwe ekamelweni lokushisa, kude nokukhanya kanye nomswakama.Umsebenzi webhayoloji: I-MOPS ivamise ukusetshenziswa njengebhafa kubhayoloji.Ibhafa ye-MOPS igcina i-pH yemidiya yamaseli ezilwane ezincelisayo.

Isicelo:Ibhafa yebhayoloji, esetshenziswa kumakhithi okuxilonga amakhemikhali ezinto eziphilayo, amakhithi e-DNA/RNA extraction kanye namakhithi okuxilonga e-PCR

Ukusetshenziswa:Izithako ku-Good's Buffer for Biological Research

I-MOPS kanye ne-coxsackievirus B3 ukuzinza

Ucwaningo lwe-coxsackievirus B3 strain 28 (CVB3/28) ukuzinza kusetshenziswa i-MOPS ukuthuthukisa ukubhafa endaweni yokuhlola yembula ukuthi i-MOPS (3-morpholinopropane-1-sulfonic acid) ikhuphule ukuzinza kwe-CVB3 futhi umphumela wawuncike ekugxiliseni.Ebangeni le-pH elingu-7.0-7.5, ukuzinza kwegciwane kuthintwa kokubili i-pH ne-MOPS.I-docking yamangqamuzana enziwe ngekhompyutha ibonise ukuthi i-MOPS ingahlala ephaketheni le-hydrophobic kuprotheni ye-capsid VP1 lapho iqembu lekhanda le-sulfonic acid lingakha amabhondi e-ionic ne-hydrogen nge-Arg95 ne-Asn211 eduze nokuvuleka kwephakethe.Imiphumela ye-MOPS kanye nokugxilwa kwe-hydrogen ion kuzinga lokubola kwegciwane kwamodela ngokufaka amapharamitha ahambisanayo kumodeli yakamuva yekinetic.Le miphumela ikhombisa ukuthi i-MOPS ingahlotshaniswa ngokuqondile ne-CVB3 futhi izinze igciwane, mhlawumbe ngokushintsha i-capsid conformational dynamics.